HDAC6 Activity Assay Kit ab284549 uses deacetylase activity of HDAC6 towards a synthetic acetylated-peptide substrate resulting in the release of an AFC fluorophore, which can be easily quantified using a conventional microplate reader.
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- Acta biochimica et biophysica Sinica 55:1987-19982023HDAC6 inhibition regulates substrate stiffness-mediated inflammation signaling in chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Zhang,Godfred K Tawiah,Yanjun Zhang,Xiaohu Wang,Xiaochun Wei,Weiyi Chen,Xiaohong Qiao,Quanyou ZhangPubMed 37644773
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Cell Lysate
- Assay type
Enzyme activity (quantitative)
Target data
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10220385). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10220385). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10220385). In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed:12024216, PubMed:20308065). Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (PubMed:30538141). Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer (PubMed:24413532). In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (PubMed:17846173). Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed:17846173).
Alternative names
Histone deacetylase 6, HD6, Tubulin-lysine deacetylase HDAC6, JM21, HDAC6, KIAA0901
What's included?
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HDAC6 Activity Assay Kit ab284549 uses deacetylase activity of HDAC6 towards a synthetic acetylated-peptide substrate resulting in the release of an AFC fluorophore, which can be easily quantified using a conventional microplate reader.
Alternative names
Histone deacetylase 6, HD6, Tubulin-lysine deacetylase HDAC6, JM21, HDAC6, KIAA0901
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Assay Platform
Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Storage information
-80°C
Notes
Histone Deacetylase 6 (HDAC6), is a class IIb HDAC enzyme that deacetylates a ε-N-acetyl lysine of histone and nonhistone protein substrates. It is a unique member of HDAC family as it contains two deacetylase domains that are proposed to function independent of each other. HDAC6 can shuttle between the nucleus and cytoplasm, suggesting potential extra-nuclear functions by regulating the acetylation status of non-histone substrates. HDAC6 also affects transcription and translation by regulating the heat-shock protein 90 (Hsp90) and stress granules.
HDAC6 Activity Assay Kit (ab284549) (K466) utilizes deacetylase activity of HDAC6 towards a synthetic acetylated-peptide substrate resulting in the release of an AFC fluorophore, which can be easily quantified using a conventional microplate reader. This simple and high-throughput adaptable assay kit can be used to detect HDAC6 activity (as low as 3 U) in complex biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K466 HDAC6 Activity Assay Kit (Fluorometric). K466-100 is the same size as the 100 test size of ab284549.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC6 or histone deacetylase 6 is a protein that primarily functions as a cytoplasmic deacetylase. It is part of the class IIb HDAC family and is known for its distinctive molecular weight of approximately 121 kDa. HDAC6 is expressed in various tissues with higher levels observed in the brain kidney and liver. This protein is unique as it contains two catalytic domains unlike other HDACs which contributes to its specific deacetylation of non-histone substrates including tubulin and Hsp90 influencing cell motility and stress response.
- Biological function summary
HDAC6 plays a significant role in processes like protein degradation and cell signaling. It is an important component of the protein quality control system involving itself in the aggresome pathway where it facilitates the removal of misfolded proteins through interaction with dynein motor proteins. In addition to its presence in the cytoplasm HDAC6 influences cell migration and immune response regulation by de-phosphorylating cortactin and affecting actin filament dynamics. Its integral role in the aggresome-autophagy pathway positions it as important for cellular homeostasis maintenance.
- Pathways
HDAC6 participates prominently in both autophagy and stress response pathways. In the autophagic process HDAC6 operates alongside ubiquitinated proteins to manage protein quality control. Moreover HDAC6 engages in stress response pathways like the heat shock response interacting directly with Hsp90 to regulate client protein activation. These pathways highlight HDAC6’s relationships with key proteins such as Hsp70 and tau linking it to cellular stress and neurodegeneration responses.
- Associated diseases and disorders
HDAC6 exhibits connections to neurodegenerative diseases and cancer. Dysregulated HDAC6 activity associates with Alzheimer's disease where it affects tau protein accumulation and degradation. The protein is also implicated in various cancers such as breast and ovarian cancer due to its influence on cell migration and invasion. It interacts with p53 impacting apoptosis and tumor progression making HDAC6 a potential target for therapeutic interventions with HDAC6 inhibitors which aim to restore normal cellular functions disrupted by abnormal HDAC6 activity.
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3 product images
Functional Studies - HDAC6 Activity Assay Kit (Fluorometric) (ab284549)
HDAC6 Activity Assay.
AFC Standard Curve. The assays were performed according to the kit protocol.
Functional Studies - HDAC6 Activity Assay Kit (Fluorometric) (ab284549)
HDAC6 Activity Assay.
HDAC6 activity with different amounts of Positive Control. The assays were performed according to the kit protocol.
Functional Studies - HDAC6 Activity Assay Kit (Fluorometric) (ab284549)
HDAC6 Activity Assay.
HDAC6 activity with different amounts of Positive Control, and in rat liver, kidney lysates along with Hela Nuclear Extract, uninduced and Camptothecin-induced Jurkat Cell Extracts are shown in the figure (n = 3). The assays were performed according to the kit protocol.
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