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AB272533

Hemoglobin Assay Kit

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(1 Publication)

Hemoglobin Assay Kit ab272533 is a simple, direct and automation-ready procedure for measuring hemoglobin concentration.

View Alternative Names

HBA2, HBA1, Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain

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Functional Studies - Hemoglobin Assay Kit (AB272533)
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Supplier Data

Functional Studies - Hemoglobin Assay Kit (AB272533)

Hemoglobin Assay Kit standard curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum, Whole Blood

Results type

Quantitative

Range

900 - 200000 µg/dL

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Product details

Hemoglobin Assay Kit (ab272533) is a simple, direct and automation-ready procedure for measuring hemoglobin concentration. This assay is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional to hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and substantially reduces interference by substances in the raw samples.

Sensitive and accurate: Linear detection range 0.9 – 200 mg/dL hemoglobin in 96-well plate assay.

Simple and high-throughput: The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

Safety: Reagents are non-toxic.

Versatility: Assays can be executed in 96-well plate or cuvet.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin also known as Hb or HBB is a protein found in red blood cells. It is a tetramer with a molecular mass of approximately 64.5 kDa composed of two alpha and two beta subunits. This protein is predominantly expressed in erythrocytes where it plays an important role in oxygen transport from the lungs to tissues and facilitates the return of carbon dioxide from tissues to the lungs for exhalation.
Biological function summary

Hemoglobin carries oxygen in blood and acts as a buffer in maintaining blood pH. Within the erythrocytes hemoglobin comprises one of the principal components of these cells each molecule binding up to four oxygen molecules. Hemoglobin exists as a complex with heme groups that directly interact with oxygen allowing hemoglobin to perform its respiratory function efficiently.

Pathways

Hemoglobin is important in the circulatory and respiratory pathways. It works in conjunction with proteins like myoglobin which assists with oxygen storage in muscles and cytochrome c which is important in the electron transport chain. Hemoglobin's function aligns with these pathways to maintain proper oxygen and energy levels throughout the body.

Hemoglobin abnormalities lead to conditions such as sickle cell anemia and thalassemia. Sickle cell anemia involves a mutation in the beta-globin gene resulting in deformed red blood cells that hinder oxygen delivery and cause pain due to blockages in blood vessels. Thalassemia results from reduced production of globin chains leading to anemia. These conditions involve aberrations in the hemoglobin or related proteins impacting overall health and requiring clinical management.
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Product protocols

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Target data

Involved in oxygen transport from the lung to the various peripheral tissues.. Hemopressin. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1 (PubMed : 18077343). Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling (PubMed : 18077343).
See full target information HBA1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal for numerical methods in biomedical engineering 39:e3671 PubMed36507614

2022

The impact of rotor configurations on hemodynamic features, hemocompatibility and dynamic balance of the centrifugal blood pump: A numerical study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Yifeng Xi,Hongyu Wang,Anqiang Sun,Xiaoyan Deng,Zengsheng Chen,Yubo Fan
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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