Hemoglobin Assay Kit (Colorimetric)

Hemoglobin Assay Kit (Colorimetric) (ab234046) provides a quick and easy method for monitoring hemoglobin levels in a wide variety of samples. In this assay, the detector selectively converts heme into a stable chemical complex that absorbs maximally at 575 nm. The intensity of the color is directly proportional to the total concentration of hemoglobin in the sample. The kit can detect as low as 0.02 g/dL hemoglobin.

This product is manufactured by BioVision, an Abcam company and was previously called K219 Hemoglobin Colorimetric Assay Kit. K219-200 is the same size as the 200 test size of ab234046.

Hemoglobin (Hb) is an iron containing globular metalloprotein found primarily in red blood cells. It carries oxygen from the lungs to the rest of the body as oxyhemoglobin and then returns back to the lungs as deoxyhemoglobin. In most vertebrates, hemoglobin is in tetrameric form comprising two α-globin chains and two β-globin chains (α₂β₂). Each chain is associated with a non-protein heme group. Aside from oxygen transport, hemoglobin can bind and transport other molecules like nitric oxide and carbon monoxide. Hemoglobin also plays an important role in maintaining the shape of the red blood cells. Alterations in the structure and binding capacity of hemoglobin are associated with many blood disorders such as sickle-cell anemia and thalassemia.