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Hexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure which can detect hexokinase activity to below 0.1 mU/well.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay time

1h

Sensitivity

< 0.1 mU/well

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What's included?

100 Test
Components
Assay Buffer LX
1 x 25 mL
Developer Solution III
1 x 1 Vial
Development Enzyme Mix IX
1 x 1 Vial
HK Coenzyme
1 x 1 Vial
HK Substrate
1 x 1 mL
Hexokinase Positive Control
1 x 1 Vial
NADH Standard I
1 x 1 Vial

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Hexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure which can detect hexokinase activity to below 0.1 mU/well.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells

Assay type

Enzyme activity (quantitative)

Reactive species

Mammals

Assay time

1h

Assay Platform

Microplate reader

Sensitivity

< 0.1 mU/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Hexokinase Activity Assay Kit (ab136957) uses a simple, sensitive and rapid procedure which can detect hexokinase activity to below 0.1 mU/well.

In the hexokinase assay protocol, glucose is converted to glucose-6-phosphate by hexokinase. The glucose-6-phosphate is then oxidized by glucose-6-phosphate dehydrogenase to form NADH. The NADH reduces a colorless probe to a colored product with strong absorbance at 450 nm.

Hexokinase activity assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze repeatedly with a microplate reader during the incubation

Technical Note: For optimal enzymatic activity samples should not contain EDTA or other chelating agents.

This product is manufactured by BioVision, an Abcam company and was previously called K789 Hexokinase Colorimetric Assay Kit. K789-100 is the same size as the 100 test size of ab136957.

Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.

    Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 1-27 fold.

  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.

    Protein concentration for samples varied from 13 mg/mL to 43 mg/mL. Samples were diluted 1-9 fold.

  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).

  • Schematic Diagram - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Schematic Diagram - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    Assay Procedure.

    In this kit glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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