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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Hexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure which can detect hexokinase activity to below 0.1 mU/well.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Colorimetric
Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells
Enzyme activity (quantitative)
Mammals
1h
< 0.1 mU/well
Hexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure which can detect hexokinase activity to below 0.1 mU/well.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Colorimetric
Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells
Enzyme activity (quantitative)
Mammals
1h
Microplate reader
< 0.1 mU/well
Blue Ice
-20°C
-20°C
-20°C
Hexokinase Activity Assay Kit (ab136957) uses a simple, sensitive and rapid procedure which can detect hexokinase activity to below 0.1 mU/well.
In the hexokinase assay protocol, glucose is converted to glucose-6-phosphate by hexokinase. The glucose-6-phosphate is then oxidized by glucose-6-phosphate dehydrogenase to form NADH. The NADH reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Hexokinase activity assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze repeatedly with a microplate reader during the incubation
Technical Note: For optimal enzymatic activity samples should not contain EDTA or other chelating agents.
This product is manufactured by BioVision, an Abcam company and was previously called K789 Hexokinase Colorimetric Assay Kit. K789-100 is the same size as the 100 test size of ab136957.
Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.
Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 1-27 fold.
Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.
Protein concentration for samples varied from 13 mg/mL to 43 mg/mL. Samples were diluted 1-9 fold.
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).
Assay Procedure.
In this kit glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com