Hexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure for the measurement of hexokinase activity. Readout on any colorimetric (450 m) plate reader.
- Complete kit including standard curve for quantitation
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Scientific reports 14:8452024Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren G Buss,Brenna A Rheinheimer,Kirsten H LimesandPubMed 38191641
- Nutrients 15:2023Effect of Moderate-Intense Training and Detraining on Glucose Metabolism, Lipid Profile, and Liver Enzymes in Male Wistar Rats: A Preclinical Randomized Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHira Shakoor,Jaleel Kizhakkayil,Mariyam Khalid,Amar Mahgoub,Carine PlatatPubMed 37686852
- Cancer research 83:3478-34912023The Ratio of Key Metabolic Transcripts Is a Predictive Biomarker of Breast Cancer Metastasis to the Lung.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepti Mathur,Chen Liao,Wendy Lin,Alessandro La Ferlita,Salvatore Alaimo,Samuel Taylor,Yi Zhong,Christine Iacobuzio-Donahue,Alfredo Ferro,Joao B XavierPubMed 37526524
- iScience 26:1073752023Increased SIRT3 combined with PARP inhibition rescues motor function of SBMA mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid R Garcia Castro,Joseph R Mazuk,Erin M Heine,Daniel Simpson,R Seth Pinches,Caroline Lozzi,Kathryn Hoffman,Phillip Morrin,Dylan Mathis,Maria V Lebedev,Elyse Nissley,Kang Hoo Han,Tyler Farmer,Diane E Merry,Qiang Tong,Maria Pennuto,Heather L MontiePubMed 37599829
- Frontiers in nutrition 10:11246782023A metabolic shift toward glycolysis enables cancer cells to maintain survival upon concomitant glutamine deprivation and V-ATPase inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlorian Lengauer,Franz Geisslinger,Antje Gabriel,Karin von Schwarzenberg,Angelika M Vollmar,Karin BartelPubMed 37255933
- Proceedings of the National Academy of Sciences of the United States of America 120:e22091771202023Mitochondrial control of microglial phagocytosis by the translocator protein and hexokinase 2 in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren H Fairley,Kei Onn Lai,Jia Hui Wong,Wei Jing Chong,Anselm Salvatore Vincent,Giuseppe D'Agostino,Xiaoting Wu,Roshan R Naik,Anusha Jayaraman,Sarah R Langley,Christiane Ruedl,Anna M BarronPubMed 36787364
- Stem cell reviews and reports 19:767-7832022Maternal Undernutrition Induces Cell Signalling and Metabolic Dysfunction in Undifferentiated Mouse Embryonic Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPooja Khurana,Andrew Cox,Barira Islam,Judith J Eckert,Sandrine Willaime-Morawek,Joanna M Gould,Neil R Smyth,Patrick C McHugh,Tom P FlemingPubMed 36517693
- Nature communications 13:67542022Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth Haythorne,Matthew Lloyd,John Walsby-Tickle,Andrei I Tarasov,Jonas Sandbrink,Idoia Portillo,Raul Terron Exposito,Gregor Sachse,Malgorzata Cyranka,Maria Rohm,Patrik Rorsman,James McCullagh,Frances M AshcroftPubMed 36376280
- Communications biology 5:11922022An organ-on-chip model of pulmonary arterial hypertension identifies a BMPR2-SOX17-prostacyclin signalling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander J Ainscough,Timothy J Smith,Maike Haensel,Christopher J Rhodes,Adam Fellows,Harry J Whitwell,Eleni Vasilaki,Kelly Gray,Adrian Freeman,Luke S Howard,John Wharton,Benjamin Dunmore,Paul D Upton,Martin R Wilkins,Joshua B Edel,Beata Wojciak-StothardPubMed 36344664
- International journal of molecular sciences 23:2022Metabolic Heterogeneity of Brain Tumor Cells of Proneural and Mesenchymal Origin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCorinna Seliger,Anne-Louise Meyer,Verena Leidgens,Lisa Rauer,Sylvia Moeckel,Birgit Jachnik,Judith Proske,Katja Dettmer,Tanja Rothhammer-Hampl,Leon D Kaulen,Markus J Riemenschneider,Peter J Oefner,Marina Kreutz,Nils-Ole Schmidt,Marsha Merrill,Martin Uhl,Kathrin Renner,Arabel Vollmann-Zwerenz,Martin Proescholdt,Peter HauPubMed 36232951
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells
- Assay type
- Enzyme activity (quantitative)
- Reactive species
- Mammals
- Assay time
- 1h
- Sensitivity
- < 0.1 mU/well
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Hexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure for the measurement of hexokinase activity. Readout on any colorimetric (450 m) plate reader.
- Complete kit including standard curve for quantitation
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells
- Assay type
- Enzyme activity (quantitative)
- Reactive species
- Mammals
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- < 0.1 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In the hexokinase assay protocol, glucose is converted to glucose-6-phosphate by hexokinase. The glucose-6-phosphate is then oxidized by glucose-6-phosphate dehydrogenase to form NADH. The NADH reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Hexokinase activity assay protocol summary:
- Add samples and standards to wells
- Add reaction mix and incubate for 20-60 min
- Analyze repeatedly with a microplate reader during the incubation
For optimal enzymatic activity samples should not contain EDTA or other chelating agents.
How other researchers are using
Hexokinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Mouse parotid salivary gland samples 1
Rat gastrocnemius muscle tissues 2
Human breast cancer cell lines 3
References:
1-Buss L et al. 2024
2-Shakoor H al. 2023
3-Mathur D et al. 2023
Related and recommended products
For fluorometric detection, we recommend Hexokinase Activity Assay Kit (Fluorometric) Hexokinase Activity Assay Kit (Fluorometric) ab211103
This product is manufactured by BioVision, an Abcam company and was previously called K789 Hexokinase Colorimetric Assay Kit. K789-100 is the same size as the 100 test size of ab136957.
Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)
Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.
Protein concentration for samples varied from 13 mg/mL to 43 mg/mL. Samples were diluted 1-9 fold.
Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)
Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.
Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 1-27 fold.
Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)
NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).
Schematic Diagram - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)
Assay Procedure.
In this kit glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
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