Hexokinase Activity Assay Kit (Colorimetric)

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Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957)

Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.

Protein concentration for samples varied from 13 mg/mL to 43 mg/mL. Samples were diluted 1-9 fold.

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Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

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Supplier Data

Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957)

NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).

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Unknown

Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957)

Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.

Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 1-27 fold.

• Schematic Diagram

Supplier Data

Schematic Diagram - Hexokinase Activity Assay Kit (Colorimetric) (AB136957)

Representative image of Hexokinase Activity Assay Kit (Colorimetric) ab136957

Components shown from left to right :

- NADH Standard I

- HK Coenzyme

- Developer Solution III

- Developer Mix G

- Hexokinase Positive Control

- HK Substrate

- Assay Buffer 60

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

• Schematic Diagram

Supplier Data

Schematic Diagram - Hexokinase Activity Assay Kit (Colorimetric) (AB136957)

Assay Procedure.

In this kit glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.