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Hexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure for the measurement of hexokinase activity. Readout on any colorimetric (450 m) plate reader.

- Complete kit including standard curve for quantitation
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957), expandable thumbnail
  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957), expandable thumbnail
  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957), expandable thumbnail
  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (AB136957), expandable thumbnail
  • Schematic Diagram - Hexokinase Activity Assay Kit (Colorimetric) (AB136957), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells
Assay type
Enzyme activity (quantitative)
Reactive species
Mammals
Assay time
1h
Sensitivity
< 0.1 mU/well

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

100 Test
Components
Assay Buffer 60
1 x 25 mL
Developer Mix G
1 x 1 Each
Developer Solution III
1 x 1 Each
HK Coenzyme
1 x 1 Each
HK Substrate
1 x 1 mL
Hexokinase Positive Control
1 x 1 Vial
NADH Standard I
1 x 1 Vial

Recommended products

Hexokinase Activity Assay Kit ab136957 uses a simple, sensitive and rapid procedure for the measurement of hexokinase activity. Readout on any colorimetric (450 m) plate reader.

- Complete kit including standard curve for quantitation
- Cited in > 50 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Tissue Extracts, Serum, Adherent cells
Assay type
Enzyme activity (quantitative)
Reactive species
Mammals
Assay time
1h
Assay Platform
Microplate reader
Sensitivity
< 0.1 mU/well

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

How the assay works

In the hexokinase assay protocol, glucose is converted to glucose-6-phosphate by hexokinase. The glucose-6-phosphate is then oxidized by glucose-6-phosphate dehydrogenase to form NADH. The NADH reduces a colorless probe to a colored product with strong absorbance at 450 nm.

Hexokinase activity assay protocol summary:

- Add samples and standards to wells
- Add reaction mix and incubate for 20-60 min
- Analyze repeatedly with a microplate reader during the incubation

For optimal enzymatic activity samples should not contain EDTA or other chelating agents.

How other researchers are using

Hexokinase Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Mouse parotid salivary gland samples 1
Rat gastrocnemius muscle tissues 2
Human breast cancer cell lines 3
References: 

1-Buss L et al. 2024
2-Shakoor H al. 2023
3-Mathur D et al. 2023

Related and recommended products

For fluorometric detection, we recommend Hexokinase Activity Assay Kit (Fluorometric) Hexokinase Activity Assay Kit (Fluorometric) ab211103

This product is manufactured by BioVision, an Abcam company and was previously called K789 Hexokinase Colorimetric Assay Kit. K789-100 is the same size as the 100 test size of ab136957.

Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.

    Protein concentration for samples varied from 13 mg/mL to 43 mg/mL. Samples were diluted 1-9 fold.

  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.

    Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 1-27 fold.

  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Functional Studies - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).

  • Schematic Diagram - Hexokinase Activity Assay Kit (Colorimetric) (ab136957), expandable thumbnail

    Schematic Diagram - Hexokinase Activity Assay Kit (Colorimetric) (ab136957)

    Assay Procedure.

    In this kit glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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