Hexokinase Activity Assay Kit (Fluorometric)

The Hexokinase Activity Assay Kit (Fluorometric) (ab211103) provides a simple, sensitive and quick method for monitoring hexokinase (HK) activity in cells, serum, and animal or plant tissues. In this assay, HK converts glucose into glucose-6-phosphate, which in turn undergoes a series of reactions and reduces the sensitive probe to generate an intense fluorescent product that can be easily detected at Ex/Em = 535/587 nm.

This assay can detect as low as 2 μU of HK activity.

This product is manufactured by BioVision, an Abcam company and was previously called K769 PicoProbe™ Hexokinase Activity Assay Kit (Fluorometric). K769-100 is the same size as the 100 test size of ab211103.

Hexokinase (HK, 6-Phosphate glucose kinase, ATP:D-Hexose 6- Phosphotransferase, ATP-dependent hexokinase, EC 1.1.1.49) is responsible for phosphorylating hexoses (six-carbon sugars) to form hexose phosphate. Hexokinases play an important role in glucose metabolism, as glucose is the most important substrate of hexokinases. Hexokinases are found in many organisms including bacteria, plants and mammals. In mammals, there are four isoforms (HK-I, II, III and IV). HK-I, HK-II, and HK-III are referred as "low Km" because of their high affinity for glucose (Km < 1mM), while HK-IV (also known as Glucokinase) has a Km for glucose 100-fold higher and can only phosphorylate glucose when the substrate concentration is high enough.

Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis.