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AB126584

HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit

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Hypoxia and the cellular response to hypoxic environment are central topics in studies of metabolism, cancer progression and development and stem cells.

View Alternative Names

BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78

5 Images
Immunocytochemistry/ Immunofluorescence - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)

Antibody specificity demonstrated by immunocytochemistry.

Primary antibodies used in this assay kit were validated by staining HeLa cells +/- treatment with 1mM DFO (24h) and imaged by fluorescent microscopy. HIF1 alpha staining is absent in untreated cells and induced by DFO treatment. HIF1 alpha localizes to the nucleus (as seen by co-localization with the DNA stain DAPI) as expected.

Immunocytochemistry/ Immunofluorescence - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)

Antibody specificity demonstrated by immunocytochemistry.

Primary antibodies used in this assay kit were validated by staining HeLa cells +/- treatment with 1mM DFO (24h) and imaged by fluorescent microscopy. GLUT1 staining labels the cell membrane and the fluorescent intensity increases with DFO treatment.

Western blot - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)
  • WB

Supplier Data

Western blot - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)

Antibody specificity demonstrated by Western Blot.

Primary antibodies used in this assay kit were validated by Western Blot using HeLa cell lysates that had been treated with a dose titration of DFO as indicated. (A) Using HIF1 alpha band (indicated by arrow) is absent in untreated cells and induced by DFO. In the Western Blot assay, the HIF1 alpha antibody has a prominent ~60kDa background band; however background signal is absent in ICE and immunocytochemistry application. (B) Similarly, GLUT1 levels are increased by DFO treatment in a dose-dependent manner. GLUT1 can be phosphorylated and glycosylated which likely contributes to the “smeary” bands on the Western Blot membrane.

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Flow Cytometry - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)
  • Flow Cyt

Supplier Data

Flow Cytometry - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)

HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (ab126584) used in Flow Cytometry.

Flow Cytometry - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)
  • Flow Cyt

Supplier Data

Flow Cytometry - HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (AB126584)

HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit (ab126584) used in Flow Cytometry.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Reacts with

Human

Assay type

Direct

Assay Platform

Flow cytometer

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Product details

Hypoxia and the cellular response to hypoxic environment are central topics in studies of metabolism, cancer progression and development and stem cells. A key player is the transcription factor HIF1 alpha (hypoxia inducible factor 1 alpha) which is stabilized at the protein level in response to decreased oxygen tension. HIF1 alpha then promotes transcription of a number of factors that alters cellular physiology. This Hypoxic Response Human Flow Cytometry Kit provides duplexed measurements of the transcription factor HIF1 alpha and the HIF1 alpha responsive protein GLUT1.

Secondary antibodies are not included with this kit and must be purchased separately.

Positive control: DFO, ab120727 (to be bought separately).

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The protein expressed by the gene HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia, activating the transcription of over 40 genes under hypoxic conditions, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and others. These genes' protein products enhance oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A is crucial for embryonic vascularization, tumor angiogenesis, and ischemic disease pathophysiology. Its activation requires transcriptional coactivators like CREBBP and EP300, with activity enhanced by interactions with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 activates CTAD and enhances activation by NCOA1 and CREBBP. Additionally, HIF1A is involved in axonal distribution and mitochondrial transport in neurons during hypoxia. In the context of microbial infection, specifically human coronavirus SARS-CoV-2, HIF1A is necessary for glycolysis induction in monocytes, leading to a proinflammatory state, inducing expression of ACE2, cytokines, and promoting virus replication and monocyte inflammatory response. This supplementary information is collated from multiple sources and compiled automatically.
See full target information HIF1A

Additional targets

SLC2A1
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