Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) ab156064 detects HDAC activity in lysates.
Images
Publications
Filter by
- Journal of biochemical and molecular toxicology 38:e235912023Design, synthesis, and histone deacetylase inhibition study of novel 4-(2-aminoethyl) phenol derivatives.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRunesh Podili,K M Abha Mishra,Ashish S Akkewar,Sanjay Kumar,V V S Prasanna Kumari Rayala,Uttam Kulhari,Bidya D Sahu,Radhakrishnanand P,Kalyan K SethiPubMed 38037273
- Microbiology spectrum :e02766222023Host-Erythrocytic Sphingosine-1-Phosphate Regulates Histone Deacetylase Activity and Exhibits Epigenetic Control over Cell Death and Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaj Kumar Sah,Sakshi Anand,Waseem Dar,Ravi Jain,Geeta Kumari,Evanka Madan,Monika Saini,Aashima Gupta,Nishant Joshi,Rahul Singh Hada,Nutan Gupta,Soumya Pati,Shailja SinghPubMed 36744922
- International journal of molecular sciences 24:20224-Hexylresorcinol Treatment before Degumming Increases the β-Sheet Structure of Silk Sericin and BMP-2 Expression in RAW264.7 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Hae Lee,HaeYong Kweon,Ji-Hyeon Oh,Yei-Jin Kang,Dae-Won Kim,Won-Geun Yang,Weon-Sik Chae,Seong-Gon KimPubMed 36613594
- Genome biology 23:1872022The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShenli Yuan,Tao Huang,Ziyou Bao,Shiyu Wang,Xinyue Wu,Jiang Liu,Hongbin Liu,Zi-Jiang ChenPubMed 36068616
- Cancers 14:2022Givinostat-Liposomes: Anti-Tumor Effect on 2D and 3D Glioblastoma Models and Pharmacokinetics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorenzo Taiarol,Chiara Bigogno,Silvia Sesana,Marcelo Kravicz,Francesca Viale,Eleonora Pozzi,Laura Monza,Valentina Alda Carozzi,Cristina Meregalli,Silvia Valtorta,Rosa Maria Moresco,Marcus Koch,Federica Barbugian,Laura Russo,Giulio Dondio,Christian Steinkühler,Francesca RePubMed 35740641
- Frontiers in molecular biosciences 9:8018162022PaDef ( var. drymifolia), a Plant Antimicrobial Peptide, Triggers Apoptosis, and Induces Global Epigenetic Modifications on Histone 3 in an Acute Lymphoid Leukemia Cell Line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaola Jiménez-Alcántar,Rodolfo López-Gómez,Joel E López-Meza,Alejandra Ochoa-ZarzosaPubMed 35141282
- eLife 10:2021Short-chain fatty acids activate acetyltransferase p300.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSydney P Thomas,John M DenuPubMed 34677127
- International journal of molecular sciences 22:2021MS-275 (Entinostat) Promotes Radio-Sensitivity in PAX3-FOXO1 Rhabdomyosarcoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatteo Cassandri,Silvia Pomella,Alessandra Rossetti,Francesco Petragnano,Luisa Milazzo,Francesca Vulcano,Simona Camero,Silvia Codenotti,Francesca Cicchetti,Roberto Maggio,Claudio Festuccia,Giovanni Luca Gravina,Alessandro Fanzani,Francesca Megiorni,Marialuigia Catanoso,Cinzia Marchese,Vincenzo Tombolini,Franco Locatelli,Rossella Rota,Francesco MaramponPubMed 34639012
- Frontiers in veterinary science 8:7165702021Panobinostat Effectively Increases Histone Acetylation and Alters Chromatin Accessibility Landscape in Canine Embryonic Fibroblasts but Does Not Enhance Cellular Reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaryam Moshref,Maria Questa,Veronica Lopez-Cervantes,Thomas K Sears,Rachel L Greathouse,Charles K Crawford,Amir KolPubMed 34660761
- International journal of molecular sciences 22:2021Effects of 4-Hexylresorcinol on Craniofacial Growth in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIn-Song Lee,Dae-Won Kim,Ji-Hyeon Oh,Suk Keun Lee,Je-Yong Choi,Seong-Gon Kim,Tae-Woo KimPubMed 34445640
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 1h
What's included?
Recommended products
Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) ab156064 detects HDAC activity in lysates.
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 1h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) ab156064 detects HDAC activity in lysates.
The HDAC Activity Assay Kit is primarily designed for the evaluation of HDAC inhibitors using a crude HDAC fraction. Also, any cultured primary cell, cell line, or tissue homogenate can be assayed for HDAC activity with the kit if the appropriate dose of HDAC specific inhibitor e.g. Trichostatin A is used.
The HDAC activity assay protocol is based on an acetylated peptide which is conjugated to AMC. AMC is a fluorescent dye and its fluoresence is quenched when conjugated to the peptide. When the HDAC de-acetylates the peptide, it becomes susceptible to cleavage by an enzyme (the Developer component). This then releases free AMC, which can be measured using a fluorescence microplate reader (Ex/Em 355/460 nm).
The HDAC assay has been shown to detect the activity of the HDAC family, at least class I HDACs in Human or animal cell lysates or in column fractions. The assay shows good linearity of sample response. The assay may be used to follow the purification of HDACs or may be used to detect the presence of HDACs in cell lysates.
Applications for this kit include:
1. Monitoring the purification of HDACs including HDAC1, 2, 3 and 8.
2. Screening inhibitors or activators of HDACs.
3. Detecting the effects of pharmacological agents on HDACs.
Background information on HDACs
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC1 also known as Histone Deacetylase 1 is a member of the histone deacetylase family with a molecular weight of approximately 55 kDa. Mechanically HDAC1 removes acetyl groups from lysine residues on histone proteins an action known as histone deacetylation. This process causes chromatin structure to become more compact which leads to transcriptional repression. HDAC1 is broadly expressed in various tissues particularly in the brain heart and kidneys and is vital for cellular development and differentiation.
- Biological function summary
The enzymatic activity of histone deacetylase effectively controls gene expression. HDAC1 participates as a part of the multiprotein complexes including SIN3 and NuRD which play vital roles in the regulation of transcription. By altering the acetylation state of histones HDAC1 influences chromatin remodeling thereby affecting the accessibility of transcription factors to DNA and controlling genes necessary for cell cycle progression and proliferation.
- Pathways
The function of HDAC1 fits into the regulation of the cell cycle and apoptosis pathways. In the cell cycle pathway HDAC1 interacts with other histone deacetylases (HDACs) and plays a role in controlling the progression of the cell division. The interplay between HDAC1 and proteins such as p53 further showcases its regulatory activity in apoptosis ensuring cell survival or programmed cell death when necessary.
- Associated diseases and disorders
HDAC1 shows significant relevance to cancer and neurodegenerative diseases. In cancer the overexpression or abnormal regulation of HDAC1 can lead to uncontrolled cell proliferation often linked to the silencing of tumor suppressor genes. Within neurodegenerative conditions HDAC1-related disturbances in gene expression may result in impaired neuronal function and survival. The involvement of HDAC1 with proteins such as p53 and other HDACs illustrates its impact on complex disease mechanisms making it a critical target for therapeutic interventions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Li, Meng et al., Frontiers in pharmacology vol. 9 533., Fig 4, doi:10.3389/fphar.2018.00533Functional Studies - Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
Butyrate, propionate, and TSA inhibited HDACs activity in HUVEC. HDACs activity were inhibited in HUVEC by treatment of butyrate and propionate. The results were normalized using the control as 100%.
Functional Studies - Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
8 uL (corresponding to 6.7e6 cells) of nuclear extracts (Nuclear Extraction Kit ab113474) from HL60 cells were assessed kinetically (+/- SD).
Functional Studies - Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
Different volumes of positive control, with inhibitor (TA), kinetically read (+/- SD).
Functional Studies - Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
Measurement of HeLa cell endogenous HDAC1 in an immunoprecipitated sample with anti-HDAC1 antibody and following the Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064) protocol.
Functional Studies - Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
Measurement of HeLa cell endogenous HDAC2 in an immunoprecipitated sample with anti-HDAC2 antibody following the Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064) protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com