JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB115106

Histone H3 (acetyl K14) Quantification Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(0 Publication)

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K14ac

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Sensitivity

> 2 ng/well

Range

20 - 5000 ng/well

Assay time

2h 30m

Assay Platform

Microplate reader

style
left-column

Complementary Products

Assay Kits

AB115091

Histone H3 Total Quantification Kit (Colorimetric)

Functional Studies - Histone H3 Total Quantification Kit (Colorimetric) (AB115091)

  • 0
  • 0
Primary Antibodies

AB1220

Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade

ChIP - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (AB1220)

  • 5
  • 72
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

  • 4
  • 6
style
left-column

Product details

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Lysine 14 at histone H3, along with K9 and K18, are primary acetylated sites of histone H3. Histone H3 (acetyl K14) is tightly involved in cell cycle regulation, cell proliferation and apoptosis. It also correlates with transcription activation.

Abcam's Histone H3 (acetyl K14) Quantification Kit (Colorimetric) (ab115106) allows the user to measure global histone H3K14 acetylation. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3K14 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab115106-96Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Antibody Buffer", "number":"AB115106-CMP05" }, { "size":"1 x 10 µL", "name":"Detection Antibody, 1 mg/mL", "number":"AB115106-CMP06" }, { "size":"3 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115106-CMP02" }, { "size":"9 x 1 Unit", "name":"8-Well Sample Strips (with Frame)", "number":"AB115106-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB115106-CMP03" }, { "size":"1 x 20 µL", "name":"Standard Control, 100 µg/mL", "number":"AB115106-CMP07" }, { "size":"1 x 10 mL", "name":"Color Developer", "number":"AB115106-CMP04" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB115106-CMP08" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab115106-48Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"10X Wash Buffer", "number":"AB115106-CMP03", "productcode":"" }, { "size":"1 x 10 µL", "name":"Standard Control, 100 µg/mL", "number":"AB115106-CMP07", "productcode":"" }, { "size":"4 x 1 Unit", "name":"8-Well Sample Strips (with Frame)", "number":"AB115106-CMP01", "productcode":"" }, { "size":"1 x 5 µL", "name":"Detection Antibody, 1 mg/mL", "number":"AB115106-CMP06", "productcode":"" }, { "size":"2 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115106-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Buffer", "number":"AB115106-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"Color Developer", "number":"AB115106-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"Stop Solution", "number":"AB115106-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 acetyl K14
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com