Skip to main content

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

Be the first to review this product! Submit a review

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Reactive species
Mouse, Human
Range
20 - 5000 ng/well
Assay time
2h 30m
Sensitivity
> 2 ng/well

Target data

Function

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

What's included?

96 Test
Components
10X Wash Buffer
1 x 20 mL
8-Well Sample Strips (with Frame)
9 x 1 Unit
8-Well Standard Control Strips
3 x 1 Unit
Antibody Buffer
1 x 12 mL
Color Developer
1 x 10 mL
Detection Antibody, 1 mg/mL
1 x 10 µL
Standard Control, 100 µg/mL
1 x 20 µL
Stop Solution
1 x 6 mL

Recommended products

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Reactive species
Mouse, Human
Range
20 - 5000 ng/well
Assay time
2h 30m
Assay Platform
Microplate reader
Sensitivity
> 2 ng/well

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Lysine 14 at histone H3, along with K9 and K18, are primary acetylated sites of histone H3. Histone H3 (acetyl K14) is tightly involved in cell cycle regulation, cell proliferation and apoptosis. It also correlates with transcription activation.

Abcam's Histone H3 (acetyl K14) Quantification Kit (Colorimetric) (ab115106) allows the user to measure global histone H3K14 acetylation. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3K14 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com