Histone H3 (acetyl K14) Quantification Kit (Colorimetric) (ab115106)

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

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Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3E, H3FD, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1, H3FI, H3C7, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, HIST1H3H, H3K14ac

Detection method: Colorimetric
Sample types: Tissue, Suspension cells, Adherent cells
Reactive species: Mouse, Human
Range: 20 - 5000 ng/well
Assay time: 2h 30m
Sensitivity: > 2 ng/well

Target data

H31_HUMAN acetyl K14

What's included?

96 Test

Components

10X Wash Buffer

1 x 20 mL

8-Well Sample Strips (with Frame)

9 x 1 Unit

8-Well Standard Control Strips

3 x 1 Unit

Antibody Buffer

1 x 12 mL

Color Developer

1 x 10 mL

Detection Antibody, 1 mg/mL

1 x 10 µL

Standard Control, 100 µg/mL

1 x 20 µL

Stop Solution

1 x 6 mL

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Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

Key facts

Detection method: Colorimetric
Sample types: Tissue, Suspension cells, Adherent cells
Reactive species: Mouse, Human
Range: 20 - 5000 ng/well
Assay time: 2h 30m
Assay Platform: Microplate reader
Sensitivity: > 2 ng/well

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: Multi
Appropriate long-term storage conditions: Multi
Storage information: Please refer to protocols

Notes

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Lysine 14 at histone H3, along with K9 and K18, are primary acetylated sites of histone H3. Histone H3 (acetyl K14) is tightly involved in cell cycle regulation, cell proliferation and apoptosis. It also correlates with transcription activation.

Abcam's Histone H3 (acetyl K14) Quantification Kit (Colorimetric) (ab115106) allows the user to measure global histone H3K14 acetylation. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3K14 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

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Product protocols

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Histone H3 (acetyl K14) Quantification Kit (Colorimetric)

What's included?

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