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AB115104

Histone H3 (acetyl K9) Quantification Kit (Colorimetric)

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(1 Publication)

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9ac

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Sensitivity

> 2 ng/well

Range

20 - 5000 ng/well

Assay time

2h 30m

Assay Platform

Microplate reader

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Product details

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 acetylation. Lysine 9 at histone H3 is a primary acetylated site of histone H3. Histone H3 (acetyl K9) appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Histone H3 (acetyl K9) is an active marker as well as being tightly involved in cell cycle regulation, cell proliferation and apoptosis. The balance between H3 (acetyl K9) and H3 (methyl K9) is important for the establishment of specific chromatin structures.

Histone H3 (acetyl K9) Quantification Kit (Colorimetric) (ab115104) allows the user to measure global acetylation of histone H3K9. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3K9 acetylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 acetyl K9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37830582

2023

Normalization of the ATP1A1 Signalosome Rescinds Epigenetic Modifications and Induces Cell Autophagy in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pradeep Kumar Rajan,Utibe-Abasi S Udoh,Yuto Nakafuku,Sandrine V Pierre,Juan Sanabria
View all publications
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websiteProtocolBooklet
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