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AB115102

Histone H3 Acetylation Assay Kit

4

(2 Reviews)

|

(7 Publications)

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.
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ELISA - Histone H3 Acetylation Assay Kit (AB115102)
  • ELISA

Supplier Data

ELISA - Histone H3 Acetylation Assay Kit (AB115102)

Acetylated histone H3 control was bound to microplate wells at different concentrations and measured using ab115102 Histone H3 Acetylation Assay Kit.

Key facts

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Assay type

Cell-based

Results type

Quantitative

Sensitivity

= 1 ng

Range

1 - 100 ng

Assay time

5h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. An imbalance in the equilibrium of histone acetylation has been associated with tumorigenesis and cancer progression.

Histone H3 Acetylation Assay Kit (ab115102) allows the user to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab115102-96Test", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Blocking Buffer", "number":"AB115102-CMP04" }, { "size":"1 x 1 mL", "name":"Histone Buffer", "number":"AB115102-CMP10" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB115102-CMP06" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB115102-CMP11" }, { "size":"1 x 28 mL", "name":"10X Wash Buffer", "number":"AB115102-CMP02" }, { "size":"1 x 12 mL", "name":"Antibody Buffer", "number":"AB115102-CMP03" }, { "size":"1 x 10 mL", "name":"10X Lysis Buffer", "number":"AB115102-CMP01" }, { "size":"1 x 10 mL", "name":"Developing Solution", "number":"AB115102-CMP08" }, { "size":"1 x 16 mL", "name":"Extraction Buffer", "number":"AB115102-CMP09" }, { "size":"1 x 20 µL", "name":"Acetylated Histone H3 Control (60 µg/mL)", "number":"AB115102-CMP12" }, { "size":"1 x 50 µL", "name":"Capture Antibody, 100 µg/mL", "number":"AB115102-CMP05" }, { "size":"1 x 20 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB115102-CMP07" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab115102-48Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Blocking Buffer", "number":"AB115102-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"10X Lysis Buffer", "number":"AB115102-CMP01", "productcode":"" }, { "size":"1 x 0.5 mL", "name":"Histone Buffer", "number":"AB115102-CMP10", "productcode":"" }, { "size":"1 x 5 mL", "name":"Developing Solution", "number":"AB115102-CMP08", "productcode":"" }, { "size":"1 x 14 mL", "name":"10X Wash Buffer", "number":"AB115102-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Extraction Buffer", "number":"AB115102-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"Stop Solution", "number":"AB115102-CMP11", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB115102-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Buffer", "number":"AB115102-CMP03", "productcode":"" }, { "size":"1 x 25 µL", "name":"Capture Antibody, 100 µg/mL", "number":"AB115102-CMP05", "productcode":"" }, { "size":"1 x 10 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB115102-CMP07", "productcode":"" }, { "size":"1 x 10 µL", "name":"Acetylated Histone H3 Control (60 µg/mL)", "number":"AB115102-CMP12", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 8:447 PubMed36335090

2022

Epigenetic mechanisms of Strip2 in differentiation of pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sureshkumar Perumal Srinivasan,Harshal Nemade,Anna Cherianidou,Luying Peng,Sara Cruz-Molina,Alvaro Rada-Iglesias,Agapios Sachinidis

The Journal of experimental medicine 219: PubMed36129517

2022

Sleep exerts lasting effects on hematopoietic stem cell function and diversity.

Applications

Unspecified application

Species

Unspecified reactive species

Cameron S McAlpine,Máté G Kiss,Faris M Zuraikat,David Cheek,Giulia Schiroli,Hajera Amatullah,Pacific Huynh,Mehreen Z Bhatti,Lai-Ping Wong,Abi G Yates,Wolfram C Poller,John E Mindur,Christopher T Chan,Henrike Janssen,Jeffrey Downey,Sumnima Singh,Ruslan I Sadreyev,Matthias Nahrendorf,Kate L Jeffrey,David T Scadden,Kamila Naxerova,Marie-Pierre St-Onge,Filip K Swirski

Scientific reports 12:7491 PubMed35523797

2022

Butyrate administration is not sufficient to improve immune reconstitution in antiretroviral-treated SIV-infected macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Ortiz,Jennifer Simpson,Charlotte A Langner,Phillip J Baker,Cynthia Aguilar,Kelsie Brooks,Jacob K Flynn,Carol L Vinton,Andrew R Rahmberg,Heather D Hickman,Jason M Brenchley

Nature communications 12:2398 PubMed33893278

2021

Arginine is an epigenetic regulator targeting TEAD4 to modulate OXPHOS in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Lin Chen,Sheng-Chieh Hsu,Tan-Ya Chung,Cheng-Ying Chu,Hung-Jung Wang,Pei-Wen Hsiao,Shauh-Der Yeh,David K Ann,Yun Yen,Hsing-Jien Kung

The Journal of investigative dermatology 141:95-104.e2 PubMed32544478

2020

Induction of Regulatory T Cells and Correction of Cytokine Disbalance by Short-Chain Fatty Acids: Implications for Psoriasis Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Agatha Schwarz,Rebecca Philippsen,Thomas Schwarz

The Journal of biological chemistry 294:8676-8689 PubMed30975903

2019

Nuclear transport of nicotinamide phosphoribosyltransferase is cell cycle-dependent in mammalian cells, and its inhibition slows cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Petr Svoboda,Edita Krizova,Sarka Sestakova,Kamila Vapenkova,Zdenek Knejzlik,Silvie Rimpelova,Diana Rayova,Nikol Volfova,Ivana Krizova,Michaela Rumlova,David Sykora,Rene Kizek,Martin Haluzik,Vaclav Zidek,Jarmila Zidkova,Vojtech Skop

Environmental pollution (Barking, Essex : 1987) 248:368-379 PubMed30818116

2019

Male exposure to bisphenol a impairs spermatogenesis and triggers histone hyperacetylation in zebrafish testes.

Applications

Unspecified application

Species

Unspecified reactive species

S González-Rojo,M Lombó,C Fernández-Díez,M P Herráez
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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