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Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes.

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Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mouse, Human
Assay time
2h 30m

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

What's included?

96 Test
Components
10X Wash Buffer
1 x 20 mL
8-Well Sample Strips (with Frame)
9 x 1 Unit
8-Well Standard Control Strips
3 x 1 Unit
Antibody Buffer
1 x 12 mL
Color Developer
1 x 10 mL
Detection Antibody, 1 mg/mL
1 x 10 µL
Standard Control, 100 µg/mL
1 x 20 µL
Stop Solution
1 x 6 mL

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Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes.

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mouse, Human
Assay time
2h 30m
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes. However, when present within protein-coding regions it prevents inappropriate transcriptional initiation of intragenic sequences. In particular, H3 (di-methyl K36) is associated with transcriptionally active genes.

Histone H3 (di-methyl K36) Quantification Kit (Colorimetric) (ab115077) allows the user to specifically measure global di-methylation of histone H3K36 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

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