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AB115077

Histone H3 (di-methyl K36) Quantification Kit (Colorimetric)

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Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K36me2, H3K36me, H3K36

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Results type

Quantitative

Assay time

2h 30m

Assay Platform

Microplate reader

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Secondary Antibodies

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Product details

Methylation of histone H3 at lysine 36 seems to be coupled to the proces of active transcriptional elongation and it is enriched towards the 3' end of target genes. However, when present within protein-coding regions it prevents inappropriate transcriptional initiation of intragenic sequences. In particular, H3 (di-methyl K36) is associated with transcriptionally active genes.

Histone H3 (di-methyl K36) Quantification Kit (Colorimetric) (ab115077) allows the user to specifically measure global di-methylation of histone H3K36 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 di methyl K36
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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