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Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3K27).

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Images

Functional Studies - Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (AB113454), expandable thumbnail

Publications

Key facts

Sample types
Tissue Extracts, Cell Lysate
Assay type
Quantitative
Reactive species
Mouse, Human
Assay time
3h

Target data

What's included?

96 Test
Components
10X Wash Buffer
1 x 22 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Adomet
1 x 50 µL
Biotinylated Substrate, 25 µg/mL
1 x 200 µL
Capture Antibody, 100 µg/mL
1 x 50 µL
Control Enzyme (300 µg/ml)
1 x 10 µL
Detection Antibody, 100 µg/mL
1 x 20 µL
Developing Solution
1 x 12 mL
HMT Standard, 10 µg/mL
1 x 20 µL
Histone Assay Buffer
1 x 3 mL
Stop Solution
1 x 6 mL

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Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3K27).

Key facts

Sample types
Tissue Extracts, Cell Lysate
Assay type
Quantitative
Reactive species
Mouse, Human
Assay time
3h
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.

Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. In mammalian cells, the EZ family group of proteins among others are responsible for the addition of one, two or three methyl groups at lysine 17 of histone H3.

Product promise

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1 product image

  • Functional Studies - Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454), expandable thumbnail

    Functional Studies - Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454)

    Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit ab113474 and H3K27 specific histone methyltrasferase activity was measured.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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