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AB113454

Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit

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(2 Publications)

Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3K27).
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Functional Studies - Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (AB113454)
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Supplier Data

Functional Studies - Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (AB113454)

Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3K27 specific histone methyltrasferase activity was measured.

Key facts

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Mouse, Human

Results type

Quantitative

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454) allows the user to measure the activity or inhibition of individual histone methyltransferase that specifically target histone H3 at lysine 27 (H3K27). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.

Methylation of histone H3 at lysine 27 (K27) is a mark of gene inactivation and it is thought to mediate heterochromatin formation by forming a binding site for Polycomb. In mammalian cells, the EZ family group of proteins among others are responsible for the addition of one, two or three methyl groups at lysine 17 of histone H3.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab113454-96Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Developing Solution", "number":"AB113454-CMP08" }, { "size":"1 x 50 µL", "name":"Capture Antibody, 100 µg/mL", "number":"AB113454-CMP06" }, { "size":"1 x 22 mL", "name":"10X Wash Buffer", "number":"AB113454-CMP03" }, { "size":"1 x 20 µL", "name":"HMT Standard, 10 µg/mL", "number":"AB113454-CMP10" }, { "size":"1 x 200 µL", "name":"Biotinylated Substrate, 25 µg/mL", "number":"AB113454-CMP05" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113454-CMP01" }, { "size":"1 x 10 µL", "name":"Control Enzyme (300 µg/ml)", "number":"AB113454-CMP02" }, { "size":"1 x 3 mL", "name":"Histone Assay Buffer", "number":"AB113454-CMP09" }, { "size":"1 x 20 µL", "name":"Detection Antibody, 100 µg/mL", "number":"AB113454-CMP07" }, { "size":"1 x 50 µL", "name":"Adomet", "number":"AB113454-CMP04" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB113454-CMP11" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab113454-48Test", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"Stop Solution", "number":"AB113454-CMP11", "productcode":"" }, { "size":"1 x 11 mL", "name":"10X Wash Buffer", "number":"AB113454-CMP03", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113454-CMP01", "productcode":"" }, { "size":"1 x 25 µL", "name":"Capture Antibody, 100 µg/mL", "number":"AB113454-CMP06", "productcode":"" }, { "size":"1 x 5 µL", "name":"Control Enzyme (300 µg/ml)", "number":"AB113454-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"HMT Standard, 10 µg/mL", "number":"AB113454-CMP10", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Histone Assay Buffer", "number":"AB113454-CMP09", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated Substrate, 25 µg/mL", "number":"AB113454-CMP05", "productcode":"" }, { "size":"1 x 10 µL", "name":"Detection Antibody, 100 µg/mL", "number":"AB113454-CMP07", "productcode":"" }, { "size":"1 x 25 µL", "name":"Adomet", "number":"AB113454-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Developing Solution", "number":"AB113454-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information Histone H3 (K27) Methyltransferase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70498 PubMed40159638

2025

Kaempferol Targets Global Epigenetic Modifiers to Impedes Growth and Migratory Ability of HeLa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nazia Afroze,Shafiul Haque,Arif Hussain

Methods in molecular biology (Clifton, N.J.) 1238:511-21 PubMed25421677

2014

Epigenetic approaches in glioblastoma multiforme and their implication in screening and diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Gwyneth Hyman,Vinod Manglik,Jeffrey M Rousch,Mukesh Verma,David Kinkebiel,Hirendra Nath Banerjee
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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