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Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites.


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Functional Studies - Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (AB113453), expandable thumbnail

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Key facts

Sample types
Tissue Extracts, Cell Lysate
Assay type
Quantitative
Reactive species
Mouse, Human
Assay time
3h

Target data

What's included?

96 Test
Components
10X Wash Buffer
1 x 22 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Adomet
1 x 50 µL
Biotinylated Substrate, 25 µg/mL
1 x 200 µL
Capture Antibody, 100 µg/mL
1 x 50 µL
Control Enzyme (300 µg/mL)
1 x 10 µL
Detection Antibody, 200 µg/mL
1 x 20 µL
Developing Solution
1 x 12 mL
HMT Standard, 10 µg/mL
1 x 20 µL
Histone Assay Buffer
1 x 3 mL
Stop Solution
1 x 6 mL

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Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites.

Key facts

Sample types
Tissue Extracts, Cell Lysate
Assay type
Quantitative
Reactive species
Mouse, Human
Assay time
3h
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.

Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3K9). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.

ab113453 recognizes methylated H3 at lysine site 9.

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1 product image

  • Functional Studies - Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453), expandable thumbnail

    Functional Studies - Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453)

    Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit ab113474 and H3-K9 specific histone methyltrasferase activity (G9a) was measured. Purified G9a was spiked into the nuclear extract that has low concentration of nuclear proteins by dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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