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AB113453

Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit

5

(1 Review)

|

(2 Publications)

Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites.
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Functional Studies - Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (AB113453)
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Supplier Data

Functional Studies - Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (AB113453)

Nuclear extracts were prepared from MCF-7 cells using the ab113474 and H3-K9 specific histone methyltrasferase activity (G9a) was measured. Purified G9a was spiked into the nuclear extract that has low concentration of nuclear proteins by dilution.

Key facts

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Mouse, Human

Results type

Quantitative

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Methylation of histone H3 at lysine 9 (K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing expression at euchromatic sites. In mammalian cells, histone lysine methyltransferases such as SuVar39h and ESET among others are responsible for methylation of lysine 9 at histone H3.

Histone H3 (K9) Methyltransferase Activity Quantification Assay Kit (ab113453) allows the user to measure the activity or inhibition of individual histone methyltransferase (HMT) that specifically target histone H3 at lysine 9 (H3K9). The kit is ready-to-use and provides all the essential components needed to carry out a successful HMT activity/inhibition quantification experiment without the need for radioactivity or any special equipment.

ab113453 recognizes methylated H3 at lysine site 9.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information Histone H3 (K9) Methyltransferase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 8:447 PubMed36335090

2022

Epigenetic mechanisms of Strip2 in differentiation of pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sureshkumar Perumal Srinivasan,Harshal Nemade,Anna Cherianidou,Luying Peng,Sara Cruz-Molina,Alvaro Rada-Iglesias,Agapios Sachinidis

Gastroenterology 159:682-696.e13 PubMed32360551

2020

SETDB1 Inhibits p53-Mediated Apoptosis and Is Required for Formation of Pancreatic Ductal Adenocarcinomas in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Ogawa,Akihisa Fukuda,Yoshihide Matsumoto,Yuta Hanyu,Makoto Sono,Yuichi Fukunaga,Tomonori Masuda,Osamu Araki,Munemasa Nagao,Takaaki Yoshikawa,Norihiro Goto,Yukiko Hiramatsu,Motoyuki Tsuda,Takahisa Maruno,Yuki Nakanishi,Mohammed S Hussein,Tatsuaki Tsuruyama,Kyoichi Takaori,Shinji Uemoto,Hiroshi Seno
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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