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AB185910

Histone H3 Modification Multiplex Assay Kit (Colorimetric)

5

(1 Review)

|

(7 Publications)

Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader.

View Alternative Names

H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B, H3F3B, H3-3A, Histone H3.3

1 Images
ELISA - Histone H3 Modification Multiplex Assay Kit (Colorimetric) (AB185910)
  • ELISA

Supplier Data

ELISA - Histone H3 Modification Multiplex Assay Kit (Colorimetric) (AB185910)

Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Rat, Human

Results type

Quantitative

Sensitivity

= 0.5 ng/well

Range

20 - 500 ng/well

Assay time

2h 30m

Assay Platform

Microplate reader

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Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

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Product details

Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader.
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3-3A
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Bone research 13:50 PubMed40335469

2025

Sirt6 deficiency promotes senescence and age-associated intervertebral disc degeneration in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pranay Ramteke,Bahiyah Watson,Mallory Toci,Victoria A Tran,Shira Johnston,Maria Tsingas,Ruteja A Barve,Ramkrishna Mitra,Richard F Loeser,John A Collins,Makarand V Risbud

Journal of cellular and molecular medicine 29:e70498 PubMed40159638

2025

Kaempferol Targets Global Epigenetic Modifiers to Impedes Growth and Migratory Ability of HeLa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nazia Afroze,Shafiul Haque,Arif Hussain

Communications biology 7:1589 PubMed39609620

2024

Prenatal dexamethasone exposure reduces osteoprogenitor proliferation in mice via histone modifications at the Mkp-1 gene locus.

Applications

Unspecified application

Species

Unspecified reactive species

Yongheng Xie,Jianwen Su,Mankai Yang,Zixian Liu,Te Chen,Jikun Qian,Bin Yu,Xianrong Zhang

Scientific reports 14:2949 PubMed38316918

2024

Epigenetic and transcriptional regulation of cytokine production by Plasmodium falciparum-exposed monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

David V L Romero,Thivya Balendran,Wina Hasang,Stephen J Rogerson,Elizabeth H Aitken,Adrian A Achuthan

International journal of molecular medicine 49: PubMed35293595

2022

Impact of on chromatin structure and histone methylation in malignant melanoma.

Applications

Unspecified application

Species

Bacteria

Mandy Schott,Melanie Kappelmann-Fenzl,Stefan Fischer,Maite G Fernandez-Barrena,Antonio Pineda-Lucena,Matías A Ávila,Silke Kuphal,Anja-Katrin Bosserhoff

Frontiers in genetics 12:768130 PubMed35096000

2022

Chrysin Modulates Aberrant Epigenetic Variations and Hampers Migratory Behavior of Human Cervical (HeLa) Cells.

Applications

Unspecified application

Species

Bacteria

Ritu Raina,Abdulmajeed G Almutary,Sali Abubaker Bagabir,Nazia Afroze,Sharmila Fagoonee,Shafiul Haque,Arif Hussain

Journal of proteomics 133:125-133 PubMed26721445

2016

HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases.

Applications

Unspecified application

Species

Bacteria

Ryan Lillico,Marina Gomez Sobral,Nicholas Stesco,Ted M Lakowski
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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