The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader.
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- International journal of molecular medicine 49:2022Impact of on chromatin structure and histone methylation in malignant melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMandy Schott,Melanie Kappelmann-Fenzl,Stefan Fischer,Maite G Fernandez-Barrena,Antonio Pineda-Lucena,Matías A Ávila,Silke Kuphal,Anja-Katrin BosserhoffPubMed 35293595
- Frontiers in genetics 12:7681302022Chrysin Modulates Aberrant Epigenetic Variations and Hampers Migratory Behavior of Human Cervical (HeLa) Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRitu Raina,Abdulmajeed G Almutary,Sali Abubaker Bagabir,Nazia Afroze,Sharmila Fagoonee,Shafiul Haque,Arif HussainPubMed 35096000
- Journal of proteomics 133:125-1332016HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan Lillico,Marina Gomez Sobral,Nicholas Stesco,Ted M LakowskiPubMed 26721445
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mouse, Rat, Human
- Range
- 20 - 500 ng/well
- Assay time
- 2h 30m
- Sensitivity
- = 0.5 ng/well
Associated Products
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Target data
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B, H3F3B, H3-3A, Histone H3.3
What's included?
Recommended products
The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mouse, Rat, Human
- Range
- 20 - 500 ng/well
- Assay time
- 2h 30m
- Assay Platform
- Microplate reader
- Sensitivity
- = 0.5 ng/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
ELISA - Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)
Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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