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Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions.
- Detection method
Fluorescent
- Sample types
Tissue, Suspension cells, Adherent cells
- Assay type
Quantitative
- Reactive species
Mouse, Human
- Assay time
2h 30m
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Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions.
Key facts
- Detection method
Fluorescent
- Sample types
Tissue, Suspension cells, Adherent cells
- Assay type
Quantitative
- Reactive species
Mouse, Human
- Assay time
2h 30m
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
Multi
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. H3 (mono-methyl K4) is associated with silenced euchromatin regions in the genome. H3 (mono-methyl K4) seems to accumulate at the 3' end of active genes both in yeast and mammals.
Histone H3 (mono-methyl K4) Quantification Kit (Fluorometric) (ab115055) allows the user to specifically measure global mono-methylation of histone H3-K4 fluorometrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
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