Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric) (ab115074)

Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

This product has no reviews yet! Submit a review

Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3E, H3C7, H3FD, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1, H3FI, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3K27me

Detection method: Colorimetric
Sample types: Tissue, Suspension cells, Adherent cells
Assay type: Quantitative
Reactive species: Mouse, Human
Assay time: 2h 30m

Target data

H31_HUMAN methyl K27

What's included?

96 Test

Components

10X Wash Buffer

1 x 20 mL

8-Well Sample Strips (with Frame)

1 x 9 Unit

8-Well Standard Control Strips

1 x 3 Unit

Antibody Buffer

1 x 12 mL

Color Developer

1 x 10 mL

Detection Antibody, 1 mg/mL

1 x 10 µL

Standard Control, 100 µg/mL

1 x 20 µL

Stop Solution

1 x 6 mL

Key facts

Detection method: Colorimetric
Sample types: Tissue, Suspension cells, Adherent cells
Assay type: Quantitative
Reactive species: Mouse, Human
Assay time: 2h 30m
Assay Platform: Microplate reader

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: Multi
Appropriate long-term storage conditions: Multi
Storage information: Please refer to protocols

Notes

Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. H3 (mono-methyl K27), a modification enriched at periocentromic heterochromatin, is broadly distributed throughout all euchromatic sites and participates in silencing gene expression. H3 (di-methyl K27) and H3 (tri-methyl K27) are the facultative heterochromatin mark which promotes the recruitment of PcG proteins for gene silencing.

Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric) (ab115074) allows the user to specifically measure global mono-, di-, and tri-methylation of histone H3K27 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Visit the general protocols
Visit troubleshooting

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Products

Resources

Support

About us

Histone H3 (pan-methyl K27) Quantification Kit (Colorimetric)

What's included?

Recommended products