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Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth.

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Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Reactive species
Mouse, Human
Assay time
3h
Sensitivity
= 2 ng/well

Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

What's included?

96 Test
Components
10X Wash Buffer
1 x 20 mL
8-Well Sample Strips (with frame)
8 x 1 Unit
8-Well Standard Control Strips
3 x 1 Unit
Antibody Buffer
1 x 12 mL
Color Developer
1 x 10 mL
Detection Antibody, 1 mg/mL
1 x 10 µL
Standard Control (100 µg/mL)
1 x 20 µL
Stop Solution
1 x 6 mL

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Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth.

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Reactive species
Mouse, Human
Assay time
3h
Assay Platform
Microplate reader
Sensitivity
= 2 ng/well

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. Histone H3 (phospho S10) is regulated by the cell cycle and has been used as a mitotic marker. In vitro studies have shown that H3 (phospho S10) is coupled to acetylation at the nearly K14 residue and that it is negatively impacted by H3 (methyl K9).

Histone H3 (phospho S10) Assay Kit (Colorimetric) (ab115127) allows the user to measure global histone H3 (phospho S10) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser10 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

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