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AB115127

Histone H3 (phospho S10) Assay Kit (Colorimetric)

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(1 Publication)

Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3S10p

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Sensitivity

= 2 ng/well

Assay time

3h

Assay Platform

Microplate reader

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Product details

Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. Histone H3 (phospho S10) is regulated by the cell cycle and has been used as a mitotic marker. In vitro studies have shown that H3 (phospho S10) is coupled to acetylation at the nearly K14 residue and that it is negatively impacted by H3 (methyl K9).

Histone H3 (phospho S10) Assay Kit (Colorimetric) (ab115127) allows the user to measure global histone H3 (phospho S10) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser10 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 phospho S10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 79:836-845.e7 PubMed32649884

2020

Epigenetic Regulation of DNA Repair Pathway Choice by MacroH2A1 Splice Variants Ensures Genome Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Robin Sebastian,Eri K Hosogane,Eric G Sun,Andy D Tran,William C Reinhold,Sandra Burkett,David M Sturgill,Prabhakar R Gudla,Yves Pommier,Mirit I Aladjem,Philipp Oberdoerffer
View all publications
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websiteProtocolBooklet
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