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AB131561

Histone H3 Total Acetylation Assay Kit (Fluorometric)

4

(3 Reviews)

|

(6 Publications)

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Assay type

Quantitative

Sensitivity

> 0.4 ng

Range

0.4 - 50 ng

Assay time

2h 30m

Assay Platform

Microplate reader

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Product details

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.

ab131561 is designed for measuring total histone H3 acetylation in a fast format. In an assay with this kit, the acetyl histone H3 is captured to the strip wells coated with an anti-acetyl histone H3 antibody. The captured acetyl histone H3 can then be detected with a labeled detection antibody followed by a fluorescent development reagent. The ratio of acetyl histone H3 is proportional to the intensity of fluorescence. The absolute amount of acetyl histone H3 can be quantified by comparing to the standard control.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Immunology 166:222-237 PubMed35285025

2022

Targeting glycolysis in Th2 cells by pterostilbene attenuates clinical severities in an asthmatic mouse model and IL-4 production in peripheral blood from asthmatic patients.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan-Teng Liu,Ying-Chyi Song,Tsai-Chen Wu,Ko-Chieh Shiung,I-Hsuan Chen,Tung-Ti Chang,Shinn-Jye Liang,Hung-Rong Yen

Frontiers in immunology 10:2745 PubMed31827471

2019

Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Infections and Thermal Stress in a Brine Shrimp () Model.

Applications

Unspecified application

Species

Unspecified reactive species

Suvra Roy,Vikash Kumar,Peter Bossier,Parisa Norouzitallab,Daisy Vanrompay

PloS one 12:e0177372 PubMed28486547

2017

HAT2 mediates histone H4K4 acetylation and affects micrococcal nuclease sensitivity of chromatin in Leishmania donovani.

Applications

Unspecified application

Species

Leishmania

Pravin K Jha,Mohd Imran Khan,Anshul Mishra,Pradeep Das,Kislay K Sinha

Environmental research 151:663-670 PubMed27619211

2016

Non-lethal heat shock increases tolerance to metal exposure in brine shrimp.

Applications

Unspecified application

Species

Unspecified reactive species

João L T Pestana,Sara C Novais,Parisa Norouzitallab,Michiel B Vandegehuchte,Peter Bossier,Karel A C De Schamphelaere

Scientific reports 6:21166 PubMed26876951

2016

Probing the phenomenon of trained immunity in invertebrates during a transgenerational study, using brine shrimp Artemia as a model system.

Applications

Unspecified application

Species

Unspecified reactive species

Parisa Norouzitallab,Kartik Baruah,Priyanka Biswas,Daisy Vanrompay,Peter Bossier

FASEB journal : official publication of the Federa 28:3552-63 PubMed24755740

2014

Environmental heat stress induces epigenetic transgenerational inheritance of robustness in parthenogenetic Artemia model.

Applications

FuncS

Species

Crayfish

Parisa Norouzitallab,Kartik Baruah,Michiel Vandegehuchte,Gilbert Van Stappen,Francesco Catania,Julie Vanden Bussche,Lynn Vanhaecke,Patrick Sorgeloos,Peter Bossier
View all publications
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