JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB115124

Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)

4

(1 Review)

|

(9 Publications)

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.
3 Images
Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)
  • FuncS

Lab

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)

Standard curve with background signal subtracted (duplicates; +/- SD).

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)
  • FuncS

Unknown

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)

The differences in H3 acetylation level in fetal livers (female fetuses aged 19 dpc) from F1, F2, and F3 generations. measured with ab113476. Histones were prepared using ab115124
Asterisk indicate a significant difference between the respective C and R groups : ∗∗ P < 0.01; NS not significant. Data presented as the means ± SE (n = 6 per group).

Nowacka-Woszuk, Joanna et al., PloS one vol. 13,2 e0193464., Fig 4, doi:10.1371/journal.pone.0193464

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)
  • FuncS

Lab

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)

Acetylation Histone 3 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 20-500 ng per well) (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Sensitivity

= 2 ng

Range

2 - 100 ng

Assay time

2h 30m

Assay Platform

Microplate reader

style
left-column

Product details

Acetylation of histones such histone H3 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H3 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. The reversible lysine acetylation of histone H3 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.
Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) allows the user to colorimetrically detect and quantify if histone H3 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab115124 is suitable for specifically measuring total histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab115124-96Test", "assetComponentDetails": [ { "size":"1 x 20 µL", "name":"Standard Control (100 µg/ml)", "number":"AB115124-CMP07" }, { "size":"3 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115124-CMP02" }, { "size":"1 x 10 µL", "name":"Detection Antibody, 1 mg/ml", "number":"AB115124-CMP06" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB115124-CMP03" }, { "size":"9 x 1 Unit", "name":"8-Well Sample Strips (with Frame)", "number":"AB115124-CMP01" }, { "size":"1 x 12 mL", "name":"Antibody Buffer", "number":"AB115124-CMP05" }, { "size":"1 x 10 mL", "name":"Color Developer", "number":"AB115124-CMP04" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB115124-CMP08" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab115124-48Test", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Buffer", "number":"AB115124-CMP05", "productcode":"" }, { "size":"1 x 5 µL", "name":"Detection Antibody, 1 mg/ml", "number":"AB115124-CMP06", "productcode":"" }, { "size":"1 x 10 µL", "name":"Standard Control (100 µg/ml)", "number":"AB115124-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115124-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"10X Wash Buffer", "number":"AB115124-CMP03", "productcode":"" }, { "size":"4 x 1 Unit", "name":"8-Well Sample Strips (with Frame)", "number":"AB115124-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Color Developer", "number":"AB115124-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"Stop Solution", "number":"AB115124-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36834533

2023

Orthodontic Compression Enhances Macrophage M2 Polarization via Histone H3 Hyperacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Wang,Sabine Groeger,Jiawen Yong,Sabine Ruf

Genes 13: PubMed36553567

2022

Pantothenate and L-Carnitine Supplementation Improves Pathological Alterations in Cellular Models of KAT6A Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Munuera-Cabeza,Mónica Álvarez-Córdoba,Juan M Suárez-Rivero,Suleva Povea-Cabello,Irene Villalón-García,Marta Talaverón-Rey,Alejandra Suárez-Carrillo,Diana Reche-López,Paula Cilleros-Holgado,Rocío Piñero-Pérez,José A Sánchez-Alcázar

Cells 11: PubMed35269485

2022

Insights into the Histone Acetylation-Mediated Regulation of the Transcription Factor Genes That Control the Embryogenic Transition in the Somatic Cells of Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Morończyk,Agnieszka Brąszewska,Barbara Wójcikowska,Karolina Chwiałkowska,Katarzyna Nowak,Anna M Wójcik,Mirosław Kwaśniewski,Małgorzata D Gaj

International journal of molecular sciences 21: PubMed32629979

2020

Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Lavinia Casati,Francesca Pagani,Roberto Maggi,Francesco Ferrucci,Valeria Sibilia

Melanoma research 28:521-526 PubMed30192303

2018

The BET-bromodomain inhibitor JQ1 mitigates vemurafenib drug resistance in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bei Zhao,Xiankui Cheng,Xiyuan Zhou

PloS one 13:e0193464 PubMed29474484

2018

Transgenerational effects of prenatal restricted diet on gene expression and histone modifications in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Nowacka-Woszuk,Izabela Szczerbal,Anna M Malinowska,Agata Chmurzynska

American journal of physiology. Lung cellular and 310:L700-10 PubMed26747785

2016

miR-185 mediates lung epithelial cell death after oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Duo Zhang,Heedoo Lee,Yong Cao,Charles S Dela Cruz,Yang Jin

PloS one 9:e107659 PubMed25222274

2014

Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows.

Applications

FuncS

Species

Cow

Guozhong Dong,Min Qiu,Changjin Ao,Jun Zhou, Khas-Erdene,Xi Wang,Zhu Zhang,You Yang

Renal failure 36:1078-82 PubMed24845033

2014

Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease.

Applications

FuncS

Species

Human

Gordana Kocic,Jovana Cukuranovic,Tatjana Jevtovic Stoimenov,Rade Cukuranovic,Vidosava Djordjevic,Dragan Bogdanovic,Vladisav Stefanovic
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

  • 4
  • 6
Assay Kits

AB115125

Histone H4 Total Acetylation Assay Kit (Colorimetric)

  • 0
  • 0
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Assay Kits

AB115102

Histone H3 Acetylation Assay Kit

ELISA - Histone H3 Acetylation Assay Kit (AB115102)

  • 4
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com