JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB115092

Histone H3 Total Quantification Kit (Fluorometric)

Be the first to review this product! Submit a review

|

(1 Publication)

Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

1 Images
Functional Studies - Histone H3 Total Quantification Kit (Fluorometric) (AB115092)
  • FuncS

Supplier Data

Functional Studies - Histone H3 Total Quantification Kit (Fluorometric) (AB115092)

A histone H3 standard curve was generated as described in the protocol of the Histone H3 Total Quantification Kit (Fluorometric) (ab115092).

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Rat, Human

Sensitivity

= 2 ng/well

Range

10 - 1000 ng/well

Assay time

2h 30m

Assay Platform

Microplate reader

style
left-column

Product details

Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.

Histone H3 Total Quantification Kit (Fluorometric) (ab115092) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.

style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab115092-96Test", "assetComponentDetails": [ { "size":"1 x 24 µL", "name":"Fluoro Enhancer", "number":"AB115092-CMP05" }, { "size":"1 x 12 mL", "name":"Antibody Buffer", "number":"AB115092-CMP06" }, { "size":"3 x 1 Unit", "name":"8 well Standard Control Strips", "number":"AB115092-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB115092-CMP03" }, { "size":"1 x 24 µL", "name":"Fluoro Developer", "number":"AB115092-CMP09" }, { "size":"9 x 1 Unit", "name":"8 well Sample Strips (with frame)", "number":"AB115092-CMP02" }, { "size":"1 x 20 µL", "name":"Standard Control (100 µg/mL)", "number":"AB115092-CMP08" }, { "size":"1 x 8 mL", "name":"Fluoro Dilution", "number":"AB115092-CMP04" }, { "size":"1 x 10 µL", "name":"Detection Antibody, 1 mg/mL", "number":"AB115092-CMP07" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab115092-48Test", "assetComponentDetails": [ { "size":"1 x 5 µL", "name":"Detection Antibody, 1 mg/mL", "number":"AB115092-CMP07", "productcode":"" }, { "size":"1 x 12 µL", "name":"Fluoro Enhancer", "number":"AB115092-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"10X Wash Buffer", "number":"AB115092-CMP03", "productcode":"" }, { "size":"1 x 4 mL", "name":"Fluoro Dilution", "number":"AB115092-CMP04", "productcode":"" }, { "size":"1 x 10 µL", "name":"Standard Control (100 µg/mL)", "number":"AB115092-CMP08", "productcode":"" }, { "size":"4 x 1 Unit", "name":"8 well Sample Strips (with frame)", "number":"AB115092-CMP02", "productcode":"" }, { "size":"2 x 1 Unit", "name":"8 well Standard Control Strips", "number":"AB115092-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Buffer", "number":"AB115092-CMP06", "productcode":"" }, { "size":"1 x 12 µL", "name":"Fluoro Developer", "number":"AB115092-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39682709

2024

Impairment of Intermediate Filament Expression Reveals Impact on Cell Functions Independent from Keratinocyte Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Klein,Imke Ramminger,Shuoqiu Bai,Thorsten Steinberg,Pascal Tomakidi
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB115072

Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric)

  • 4
  • 1
Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

  • 4
  • 6
Assay Kits

AB185914

Histone H4 Modification Multiplex Assay Kit (Colorimetric)

Functional Studies - Histone H4 Modification Multiplex Assay Kit (Colorimetric) (AB185914)

  • 5
  • 1
Assay Kits

AB185910

Histone H3 Modification Multiplex Assay Kit (Colorimetric)

ELISA - Histone H3 Modification Multiplex Assay Kit (Colorimetric) (AB185910)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com