Histone H3 Total Quantification Kit (Fluorometric) (ab115092)

Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair.

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Alternative names=Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FD, H3C7, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, HIST1H3E, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1

Detection method: Fluorescent
Sample types: Tissue, Suspension cells, Adherent cells
Reactive species: Mouse, Rat, Human
Range: 10 - 1000 ng/well
Assay time: 2h 30m
Sensitivity: = 2 ng/well

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Target data

H31_HUMAN

What's included?

96 Test

Components

10X Wash Buffer

1 x 20 mL

8 well Sample Strips (with frame)

9 x 1 Unit

8 well Standard Control Strips

3 x 1 Unit

Antibody Buffer

1 x 12 mL

Detection Antibody, 1 mg/mL

1 x 10 µL

Fluoro Developer

1 x 24 µL

Fluoro Dilution

1 x 8 mL

Fluoro Enhancer

1 x 24 µL

Standard Control (100 µg/mL)

1 x 20 µL

Key facts

Detection method: Fluorescent
Sample types: Tissue, Suspension cells, Adherent cells
Reactive species: Mouse, Rat, Human
Range: 10 - 1000 ng/well
Assay time: 2h 30m
Assay Platform: Microplate reader
Sensitivity: = 2 ng/well

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: Multi
Appropriate long-term storage conditions: Multi
Storage information: Please refer to protocols

Notes

Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.

Histone H3 Total Quantification Kit (Fluorometric) (ab115092) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.

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Product protocols

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Histone H3 Total Quantification Kit (Fluorometric)

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