JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB115072

Histone H3 (tri-methyl K27) Quantification Kit (Colorimetric)

4

(1 Review)

|

(1 Publication)

Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K27me3, H3K27me, H3K27

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Assay type

Quantitative

Sensitivity

>= 2 ng/well

Range

20 - 5000 ng/well

Assay time

2h 30m

Assay Platform

Microplate reader

style
left-column

Product details

Methylation of histone H3 at lysine 27 has an important role in transcription repression and it is catalyzed by G9a and Polycomb Groups enzymes such as EZH2. In particular, H3(try methyl K27) is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing. Increased global histone H3 (tri methyl K27) is also found to be involved in some pathological processes such as cancer progression.

Histone H3K27me3 is quantified colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab115072-96Test", "assetComponentDetails": [ { "size":"9 x 1 Unit", "name":"8-Well Sample Strips (with frame)", "number":"AB115072-CMP01" }, { "size":"1 x 10 µL", "name":"Detection Antibody, 1 mg/mL", "number":"AB115072-CMP07" }, { "size":"1 x 12 mL", "name":"Antibody Buffer", "number":"AB115072-CMP03" }, { "size":"3 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115072-CMP02" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB115072-CMP05" }, { "size":"1 x 20 µL", "name":"Standard Control (100 µg/mL)", "number":"AB115072-CMP04" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB115072-CMP08" }, { "size":"1 x 10 mL", "name":"Color Developer", "number":"AB115072-CMP06" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab115072-48Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"10X Wash Buffer", "number":"AB115072-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Buffer", "number":"AB115072-CMP03", "productcode":"" }, { "size":"4 x 1 Unit", "name":"8-Well Sample Strips (with frame)", "number":"AB115072-CMP01", "productcode":"" }, { "size":"1 x 5 µL", "name":"Detection Antibody, 1 mg/mL", "number":"AB115072-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115072-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"Standard Control (100 µg/mL)", "number":"AB115072-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"Stop Solution", "number":"AB115072-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"Color Developer", "number":"AB115072-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 tri methyl K27
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 10:2026 PubMed31507612

2019

Transcriptomic and Epigenetic Alterations in Dendritic Cells Correspond With Chronic Kidney Disease in Lupus Nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Wardowska,Michał Komorniczak,Barbara Bułło-Piontecka,M Alicja Dȩbska-Ślizień,Michał Pikuła
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB115091

Histone H3 Total Quantification Kit (Colorimetric)

Functional Studies - Histone H3 Total Quantification Kit (Colorimetric) (AB115091)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Flow Cytometry - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

  • 4
  • 6
Primary Antibodies

AB5103

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (AB5103)

  • 4
  • 28

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com