JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB115056

Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(1 Publication)

Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me3, H3K4me, H3K4

1 Images
Functional Studies - Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (AB115056)
  • FuncS

Supplier Data

Functional Studies - Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (AB115056)

Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of trimethyl-H3-K4 was measured using ab115056.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mammals

Assay type

Quantitative

Assay time

2h 30m

Assay Platform

Microplate reader

style
left-column

Product details

Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. Enrichment of H3 (tri-methyl K4) is found at transcription start sites of active genes where they contribute to transcriptional initiation.

style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab115056-96Test", "assetComponentDetails": [ { "size":"3 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115056-CMP02" }, { "size":"1 x 20 µL", "name":"Standard Control (100 µg/ml)", "number":"AB115056-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB115056-CMP03" }, { "size":"9 x 1 Unit", "name":"8-Well Sample Strips (with frame)", "number":"AB115056-CMP01" }, { "size":"1 x 12 mL", "name":"Antibody Buffer", "number":"AB115056-CMP05" }, { "size":"1 x 10 µL", "name":"Detection Antibody, 1 mg/mL", "number":"AB115056-CMP06" }, { "size":"1 x 10 mL", "name":"Color Developer", "number":"AB115056-CMP04" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB115056-CMP08" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab115056-48Test", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"8-Well Standard Control Strips", "number":"AB115056-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"Standard Control (100 µg/ml)", "number":"AB115056-CMP07", "productcode":"" }, { "size":"4 x 1 Unit", "name":"8-Well Sample Strips (with frame)", "number":"AB115056-CMP01", "productcode":"" }, { "size":"1 x 5 µL", "name":"Detection Antibody, 1 mg/mL", "number":"AB115056-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Buffer", "number":"AB115056-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"10X Wash Buffer", "number":"AB115056-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Color Developer", "number":"AB115056-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"Stop Solution", "number":"AB115056-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
See full target information H3C1 tri methyl K4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 10:2026 PubMed31507612

2019

Transcriptomic and Epigenetic Alterations in Dendritic Cells Correspond With Chronic Kidney Disease in Lupus Nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Wardowska,Michał Komorniczak,Barbara Bułło-Piontecka,M Alicja Dȩbska-Ślizień,Michał Pikuła
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB115091

Histone H3 Total Quantification Kit (Colorimetric)

Functional Studies - Histone H3 Total Quantification Kit (Colorimetric) (AB115091)

  • 0
  • 0
Assay Kits

AB185910

Histone H3 Modification Multiplex Assay Kit (Colorimetric)

ELISA - Histone H3 Modification Multiplex Assay Kit (Colorimetric) (AB185910)

  • 5
  • 1
Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

  • 4
  • 6
Assay Kits

AB113455

Histone Demethylase (H3K4) Activity Quantification Assay Kit

Functional Studies - Histone Demethylase (H3K4) Activity Quantification Assay Kit (AB113455)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com