Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.
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- Frontiers in immunology 10:20262019Transcriptomic and Epigenetic Alterations in Dendritic Cells Correspond With Chronic Kidney Disease in Lupus Nephritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Wardowska et. al.PubMed 31507612
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay time
- 2h 30m
Target data
Function
The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, HIST1H3C, H3FC, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K4me3
What's included?
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Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue, Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay time
- 2h 30m
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. Enrichment of H3 (tri-methyl K4) is found at transcription start sites of active genes where they contribute to transcriptional initiation.
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1 product image
Functional Studies - Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056)
Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (Histone Extraction Kit ab113476) and the amount of trimethyl-H3-K4 was measured using ab115056.
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