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Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.

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Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Reactive species

Mammals

Assay time

2h 30m

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Shipping To:
United States

Images

What's included?

96 Test
Components
10X Wash Buffer
1 x 20 mL
8-Well Sample Strips (with frame)
9 x 1 Unit
8-Well Standard Control Strips
3 x 1 Unit
Antibody Buffer
1 x 12 mL
Color Developer
1 x 10 mL
Detection Antibody, 1 mg/mL
1 x 10 µL
Standard Control (100 µg/ml)
1 x 20 µL
Stop Solution
1 x 6 mL

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Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.

Is this your first time purchasing this product? Get 20% off one unit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, and Australia.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Reactive species

Mammals

Assay time

2h 30m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Histone H3 (tri-methyl K4) Quantification Kit (Colorimetric) (ab115056) allows the user to specifically measure global tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.

Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. Enrichment of H3 (tri-methyl K4) is found at transcription start sites of active genes where they contribute to transcriptional initiation.

Product promise

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Full details and terms and conditions can be found here:
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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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