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AB115064

Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric)

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(2 Publications)

Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) (ab115064) allows the user to measure global tri-methylation of histone H3K9 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

View Alternative Names

H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9me3, H3K9me, H3K9

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Functional Studies - Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) (AB115064)
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Supplier Data

Functional Studies - Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) (AB115064)

Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476) and the amount of trimethyl-H3-K9 was measured using ab115064.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Results type

Quantitative

Assay time

2h 30m

Assay Platform

Microplate reader

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Complementary Products

Sample Prep & Detection Kits

AB113476

Histone Extraction Kit

Functional Studies - Histone Extraction Kit (AB113476)

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Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB115062

Histone H3 (di-methyl K9) Quantification Kit (Colorimetric)

Functional Studies - Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (AB115062)

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  • 0
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Product details

Histone H3 (tri-methyl K9) Quantification Kit (Colorimetric) (ab115064) allows the user to specifically measure global tri-methylation of histone H3K9 colorimetrically using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells

Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as heterochromatin formation. In particular, H3(tri -methyl K9) mediates heterochromatin formation by forming a binding site for HP and is a stable heterochromatin mark which promotes gene silencing.is a facultative heterochromatin mark, which promotes the recruitment of polycomb group proteins for gene silencing.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H3C1 tri methyl K9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37830582

2023

Normalization of the ATP1A1 Signalosome Rescinds Epigenetic Modifications and Induces Cell Autophagy in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pradeep Kumar Rajan,Utibe-Abasi S Udoh,Yuto Nakafuku,Sandrine V Pierre,Juan Sanabria

The Journal of neuroscience : the official journal of the Society for Neuroscience 36:3611-22 PubMed27013689

2016

H3K9me3 Inhibition Improves Memory, Promotes Spine Formation, and Increases BDNF Levels in the Aged Hippocampus.

Applications

FuncS

Species

Mouse

Shikha Snigdha,G Aleph Prieto,Arpine Petrosyan,Brad M Loertscher,André P Dieskau,Larry E Overman,Carl W Cotman
View all publications
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