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Abcam's Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914) is a complete set of optimized reagents to detect and quantify nearly all histone H4 modifications (10 different types) simultaneously in a simple, ELISA-like format with use of a standard microplate reader.

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Images

Functional Studies - Histone H4 Modification Multiplex Assay Kit (Colorimetric) (AB185914), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mouse, Rat, Human
Range
20 - 500 ng/well
Assay time
2h 30m
Sensitivity
= 0.5 ng/well

Associated Products

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Target data

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Alternative names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4

What's included?

96 Test
Components
10X Wash Buffer
1 x 28 mL
8-Well Strips
2 x 1 Unit
96-Well Strip Plate (with Frame)
1 x 1 Unit
Adhesive Covering Film
1 x 1 Unit
Antibody Buffer
1 x 8 mL
Assay Control Protein
1 x 20 µL
Detection Antibody
1 x 12 µL
Developer Solution
1 x 12 mL
Stop Solution
1 x 12 mL

Recommended products

Abcam's Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914) is a complete set of optimized reagents to detect and quantify nearly all histone H4 modifications (10 different types) simultaneously in a simple, ELISA-like format with use of a standard microplate reader.

Key facts

Detection method
Colorimetric
Sample types
Tissue, Suspension cells, Adherent cells
Assay type
Quantitative
Reactive species
Mouse, Rat, Human
Range
20 - 500 ng/well
Assay time
2h 30m
Assay Platform
Microplate reader
Sensitivity
= 0.5 ng/well

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

Abcam's Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914) is a complete set of optimized reagents to detect and quantify nearly all histone H4 modifications (10 different types) simultaneously in a simple, ELISA-like format with use of a standard microplate reader.

ab185914 is designed for measuring multiple histone H4 modifications simultaneously. In an assay with this kit, each histone H4 modified at specific sites will be captured by an antibody that is coated on the strip wells and specifically targets the appropriate histone H4 modification pattern. The captured histone modified at specific sites will be detected with a detection antibody, followed by a color development reagent. The ratio of modified histone is proportional to the intensity of absorbance measured by a microplate reader at a wavelength of 450 nm.

Starting Materials
Input materials can be histone extracts or purified histone H4 proteins obtained from human, mouse, rat, as well as a broad range of species including most plants, fungi, and bacteria, based on high sequence homology of histone H4. The amount of histone extracts for each assay can be 20 ng to 500 ng with an optimal range of 50 to 100 ng depending on the purity of histone extracts. The amount of purified histone H4 proteins for each assay can be 1 ng to 25 ng with an optimal range of 4 to 5 ng.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914), expandable thumbnail

    Functional Studies - Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914)

    Example Data:.

    Histone extracts were prepared from MCF-7 and MDA-231 cells using the Histone Extraction Kit (Histone Extraction Kit ab113476). 10 histone H4 modifications were measured using the Histone H4 Modification Multiplex Assay Kit (Colorimetric) (ab185914). 100 ng of total histone proteins per well were used.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com