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AB115125

Histone H4 Total Acetylation Assay Kit (Colorimetric)

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(4 Publications)

Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Sensitivity

= 2 ng/well

Range

5 - 2000 ng/well

Assay time

2h 30m

Assay Platform

Microplate reader

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Product details

Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 acetylation. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptional activity of eukaryotic chromatin domains. The reversible lysine acetylation of histone H4 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.

Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) enables the user to colorimetrically detect and quantify if histone H4 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. Histone H4 Total Acetylation Detection Fast Kit (Colorimetric) is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of environmental research and public health 18: PubMed34831936

2021

Sex Steroid Regulation of Oxidative Stress in Bone Cells: An In Vitro Study.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Sibilia,Daniele Bottai,Roberto Maggi,Francesca Pagani,Raffaella Chiaramonte,Domenica Giannandrea,Valentina Citro,Natalia Platonova,Lavinia Casati

International journal of molecular sciences 21: PubMed32629979

2020

Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Lavinia Casati,Francesca Pagani,Roberto Maggi,Francesco Ferrucci,Valeria Sibilia

American journal of physiology. Lung cellular and 310:L700-10 PubMed26747785

2016

miR-185 mediates lung epithelial cell death after oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Duo Zhang,Heedoo Lee,Yong Cao,Charles S Dela Cruz,Yang Jin

Renal failure 36:1078-82 PubMed24845033

2014

Global and specific histone acetylation pattern in patients with Balkan endemic nephropathy, a worldwide disease.

Applications

FuncS

Species

Human

Gordana Kocic,Jovana Cukuranovic,Tatjana Jevtovic Stoimenov,Rade Cukuranovic,Vidosava Djordjevic,Dragan Bogdanovic,Vladisav Stefanovic
View all publications
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