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AB131562

Histone H4 Total Acetylation Assay Kit (Fluorometric)

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(3 Publications)

Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Reacts with

Mouse, Human

Sensitivity

> 0.4 ng/well

Range

5 - 2000 ng/well

Assay time

2h 30m

Assay Platform

Microplate reader

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Product details

Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptional activity of eukaryotic chromatin domains. The reversible lysine acetylation of histone H4 may play a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair, nuclear import and neuronal repression.

ab131562 enables the user to fluorometrically detect and quantify if histone H4 is acetylated. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. ab131562 is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 10:2745 PubMed31827471

2019

Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Infections and Thermal Stress in a Brine Shrimp () Model.

Applications

Unspecified application

Species

Unspecified reactive species

Suvra Roy,Vikash Kumar,Peter Bossier,Parisa Norouzitallab,Daisy Vanrompay

Environmental research 151:663-670 PubMed27619211

2016

Non-lethal heat shock increases tolerance to metal exposure in brine shrimp.

Applications

Unspecified application

Species

Unspecified reactive species

João L T Pestana,Sara C Novais,Parisa Norouzitallab,Michiel B Vandegehuchte,Peter Bossier,Karel A C De Schamphelaere

Scientific reports 6:21166 PubMed26876951

2016

Probing the phenomenon of trained immunity in invertebrates during a transgenerational study, using brine shrimp Artemia as a model system.

Applications

Unspecified application

Species

Unspecified reactive species

Parisa Norouzitallab,Kartik Baruah,Priyanka Biswas,Daisy Vanrompay,Peter Bossier
View all publications
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Product promise

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