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AB156909

Histone H4 Total Quantification Kit (Colorimetric)

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(1 Publication)

Abcam's Histone H4 Total Quantification Kit (Colorimetric) is a complete set of essential components which enables an experimenter to colorimetrically quantify levels of histone H4 proteins independent of their modified states.

View Alternative Names

H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4

5 Images
Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)
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Lab

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)

Standard curve with background signal subtracted (duplicates; +/- SD).

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)
  • FuncS

Lab

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)

Total H4 per mg of extracted histones from HeLa control cells (C) or cells treated with 10 micromolar sodium butyrate (But) (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)
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Supplier Data

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)

Illustrated standard curve generated with H4 standard.

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)
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Supplier Data

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)

Histone H4 Quantification.

Histone nuclear extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the ODs generated from Histone H4 are measured.

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)
  • FuncS

Lab

Functional Studies - Histone H4 Total Quantification Kit (Colorimetric) (AB156909)

Total H4 per mg of extracted histones from murine tissue preparations (extracted using ab113476; 50-500 ng per well) (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Nuclear Extracts, Cell culture extracts, Tissue Extracts

Reacts with

Mouse, Rat, Human

Assay type

Quantitative

Sensitivity

>= 0.5 ng/well

Range

50 - 1000 ng

Assay time

3h 30m

Assay Platform

Microplate reader

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Product details

Abcam's Histone H4 Total Quantification Kit (Colorimetric) is a complete set of essential components which enables an experimenter to colorimetrically quantify levels of histone H4 proteins independent of their modified states. It can also be used for normalizing the modified histone H4 content of samples when run in parallel with Abcam's histone modification quantification kit series.

The kit is suitable for specifically measuring total histone H4 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
See full target information H4C1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Infection and immunity 90:e0049121 PubMed35130455

2022

Neutrophil Extracellular Traps as an Exacerbating Factor in Bacterial Pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan L Sanders,Ian M C Martin,Arjun Sharma,Matthew R Jones,Lee J Quinton,Markus Bosmann,Joseph P Mizgerd
View all publications
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websiteProtocolBooklet
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