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AB228550

Hoechst 33258 Staining Dye Solution

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(41 Publications)

Hoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy.
2 Images
Spectrum - Hoechst 33258 Staining Dye Solution (AB228550)
  • Spectrum

Unknown

Spectrum - Hoechst 33258 Staining Dye Solution (AB228550)
Chemical Structure - Hoechst 33258 Staining Dye Solution (AB228550)
  • Chemical Structure

Unknown

Chemical Structure - Hoechst 33258 Staining Dye Solution (AB228550)

Key facts

Sample types

Suspension cells, Adherent cells

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Fluorescence Microscopy": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Hoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy.

This product may be used in fluorescence microscopy, microplate, cuvette and flow cytometry applications. It can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve.

Molecular Weight: 533.88

Spectral Properties: Excitation = 352 nm; Fluorescence = 461 nm

The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells and are often used as a substitute for another nucleic acid stain (DAPI). The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant.

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What's included?

{ "values": { "5mL": { "sellingSize": "5 mL", "publicAssetCode":"ab228550-5mL", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"Hoechst 33258 (20 mM solution in water)", "number":"AB228550-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (41)

Recent publications for all applications. Explore the full list and refine your search

Advances in pharmacological and pharmaceutical sciences 2025:7334303 PubMed40458441

2025

André Rhizome Extract Alleviates Scopolamine-Induced Cognitive Impairment and Neuroinflammation in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Thaneeya Hawiset,Napatr Sriraksa,Shisanupong Anukanon,Utcharaporn Kamsrijai,Siwaporn Praman,Narudol Teerapattarakan,Prachak Inkaew

Nature communications 16:2185 PubMed40038329

2025

E3 ubiquitin ligase RNF128 promotes Lys63-linked polyubiquitination on SRB1 in macrophages and aggravates atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yapeng Liu,Xinyu Zhang,Liwen Yu,Lei Cao,Jie Zhang,Qian Li,Xiaohong Wang,Wenqian Qi,Liangyu Cai,Ruiqing Ren,Weiwei Wang,Xiaobin Guo,Guohai Su,Bo Xi,Yun Zhang,Chengjiang Gao,Meng Zhang,Cheng Zhang

Bio-protocol 15:e5181 PubMed39959295

2025

Development and Validation of Mouse Models for Studying Genital Tract Infection Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Wang,Zixuan Han,Luying Wang,Xin Sun,Qi Tian,Tianyuan Zhang

Molecular medicine (Cambridge, Mass.) 30:272 PubMed39719558

2024

Doublecortin regulates the mitochondrial-dependent apoptosis in glioma via Rho-A/Net-1/p38-MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Iqra Nadeem,Zhou Han,Hong Xiaoliang,Seyram Yao Adzraku,Piniel Alphayo Kambey,Kouminin Kanwore,Mu Peipei,Adebayo Oluwafemi Adekunle,Joseph Adu-Amankwaah,Abiola Abdulrahman Ayanlaja,Yi Zheng,Gao Dianshuai,Xiaomei Liu,Yuanjian Song

Scientific reports 14:26021 PubMed39472479

2024

Reverse engineering of feedforward cortical-Hippocampal microcircuits for modelling neural network function and dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Katrine Sjaastad Hanssen,Nicolai Winter-Hjelm,Salome Nora Niethammer,Asgeir Kobro-Flatmoen,Menno P Witter,Axel Sandvig,Ioanna Sandvig

Disease models & mechanisms 17: PubMed39206627

2024

Axolotl mandible regeneration occurs through mechanical gap closure and a shared regenerative program with the limb.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Kramer,Rita Aires,Sean D Keeley,Tom Alexander Schröder,Günter Lauer,Tatiana Sandoval-Guzmán

Frontiers in pharmacology 15:1412520 PubMed38895627

2024

The autophagic regulation of rosiglitazone-promoted adipocyte browning.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Li,Wanqing Zheng,Xinhang Li,Zhengwei Lue,Yun Liu,Jiaying Wu,Xiangnan Zhang

American journal of physiology. Lung cellular and molecular physiology 326:L698-L712 PubMed38591125

2024

Chronic intermittent hypoxia elicits distinct transcriptomic responses among neurons and oligodendrocytes within the brainstem of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hemalatha Bhagavan,Aguan D Wei,Luiz M Oliveira,Kimberly A Aldinger,Jan-Marino Ramirez

Seminars in plastic surgery 38:31-38 PubMed38495069

2024

H Vessel Formation as a Marker for Enhanced Bone Healing in Irradiated Distraction Osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Daniel,Nathan Sheppard,Garrison Carlos,Noah Nelson,Alex Donneys,Steven R Buchman

Cancer cell international 24:27 PubMed38200575

2024

Targeting cholesterol impairs cell invasion of all breast cancer types.

Applications

Unspecified application

Species

Unspecified reactive species

Mauriane Maja,Marie Verfaillie,Patrick Van Der Smissen,Patrick Henriet,Christophe E Pierreux,Nor Eddine Sounni,Donatienne Tyteca
View all publications
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Product promise

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