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AB242292

Homocitrulline/Citrulline Assay Kit

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(3 Publications)

Homocitrulline/Citrulline Assay Kit ab242292 provides a convenient colorimetric method for the detection of total homocitrulline/citrulline from cells, tissue, plasma, serum, or urine samples.
3 Images
Functional Studies - Homocitrulline/Citrulline Assay Kit (AB242292)
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Supplier Data

Functional Studies - Homocitrulline/Citrulline Assay Kit (AB242292)

Citrulline standard curve

Functional Studies - Homocitrulline/Citrulline Assay Kit (AB242292)
  • FuncS

Supplier Data

Functional Studies - Homocitrulline/Citrulline Assay Kit (AB242292)

Homocitrulline standard curve.

Functional Studies - Homocitrulline/Citrulline Assay Kit (AB242292)
  • FuncS

Supplier Data

Functional Studies - Homocitrulline/Citrulline Assay Kit (AB242292)

Detection of homocitrulline/citrulline in human urine.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Cell Lysate

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Product details

The Homocitrulline/Citrulline Assay Kit (ab242292) provides a convenient colorimetric method for the detection of total homocitrulline/citrulline from cells, tissue, plasma, serum, or urine samples.

The content of homocitrulline and citrulline in the unknown samples is determined by comparison with a predetermined standard curve. The provided reagents are sufficient for the evaluation of 100 assays including standards and unknown samples.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab242292-100Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"Citrulline Standard", "number":"AB242292-CMP04", "productcode":"" }, { "size":"1 x 500 µL", "name":"SDS Solution", "number":"AB242292-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Reagent A", "number":"AB242292-CMP02", "productcode":"" }, { "size":"1 x 500 µL", "name":"Proteinase K", "number":"AB242292-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Assay Reagent B", "number":"AB242292-CMP03", "productcode":"" }, { "size":"1 x 50 µL", "name":"Homocitrulline Standard", "number":"AB242292-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrulline also known as L-citrulline engages in important roles within the nitrogen balance of the body. It is a non-essential amino acid with a molecular weight of about 175.19 g/mol. Citrulline is not incorporated into proteins but is primarily found in liver and intestinal cells. It serves as an intermediate in the urea cycle an important metabolic pathway related to the disposal of excess nitrogen from the body.
Biological function summary

Citrulline plays a significant part in the body's nitrogen cycle and enhances the production of nitric oxide a vital signaling molecule. Citrulline participates in converting ammonia a byproduct from amino acid breakdown into urea in the liver which the body can then excrete. It does not belong to a complex but acts as a precursor to the amino acid arginine enhancing its levels and promoting vasodilation. Citrulline effects also extend to influencing muscle metabolism and cellular energy production.

Pathways

Citrulline participates in the urea cycle and nitric oxide synthesis pathway. It provides a link between ammonia detoxification and the synthesis of arginine. This link enhances nitric oxide availability which is essential for regulating vascular homeostasis and blood pressure. Additionally citrulline relates to the arginine biosynthesis pathway where it aids the conversion to arginine mediated by enzymes such as argininosuccinate synthetase and argininosuccinate lyase. These interactions with enzymes highlight its integration into metabolic pathways central to the organism's homeostasis.

Citrulline shows involvement in cardiovascular diseases and urea cycle disorders. Citrulline has emerged as a therapeutic marker for cardiovascular diseases by enhancing nitric oxide production and improving endothelial function. Citrulline's role in urea cycle disorders links it to conditions where the body's ability to detoxify ammonia is compromised. Furthermore citrulline's relationship with arginine impacts both cardiac health and ammonia detoxification integrating it into therapeutic strategies targeting these conditions.
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Product protocols

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Target data

See full target information Citrulline
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0289476 PubMed37527267

2023

The effects of a comparatively higher dose of 1000 mg/kg/d of oral L- or D-arginine on the L-arginine metabolic pathways in male Sprague-Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dain Raina Kim,Sarah Martin,Kaushik Desai

Journal of controlled release : official journal of the Controlled Release Society 349:929-939 PubMed35926754

2022

Camptothesome elicits immunogenic cell death to boost colorectal cancer immune checkpoint blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiren Wang,Wenpan Li,Jonghan Park,Karina Marie Gonzalez,Aaron James Scott,Jianqin Lu

The British journal of nutrition 123:135-148 PubMed31647043

2019

The effects of oral arginine on its metabolic pathways in Sprague-Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Martin,Kaushik Desai
View all publications
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