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AB133105

Human NFkB p50 Transcription Factor Assay Kit

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ab133105 Human NFkB p50 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
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Functional Studies - Human NFkB p50 Transcription Factor Assay Kit (AB133105)
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Supplier Data

Functional Studies - Human NFkB p50 Transcription Factor Assay Kit (AB133105)

Sensitivity of the NFkB assay using purified Human recombinant p50.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Microplate reader

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Product details

ab133105 Human NFkB p50 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NFkB response element is immobilized onto the bottom of wells of a 96-well plate. NFkB contained in a nuclear extract, binds specifically to the NFkB response element. NFkB (p50) is detected by addition of specific primary antibody directed against NFkB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133105 detects Human NFkB (p50). It will not cross-react with NFkB (p65).

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What's included?

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com