Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- In vivo (Athens, Greece) 38:73-812024Ginger Supplementation Attenuated Mitochondrial Fusion and Improved Skeletal Muscle Size in Type 2 Diabetic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCasey R Appell,Nigel C Jiwan,Rui Wang,Chwan-Li Shen,Hui-Ying LukPubMed 38148056
- Scientific reports 14:42792024Kallistatin deficiency exacerbates neuronal damage after cardiac arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayoung Kim,Gil Joon Suh,Woon Yong Kwon,Kyung Su Kim,Yoon Sun Jung,Taegyun Kim,Heesu ParkPubMed 38383562
- The Journal of clinical investigation 133:2023Aryl hydrocarbon receptor sulfenylation promotes glycogenolysis and rescues cancer chemoresistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNannan Zhou,Jie Chen,Zheng Ling,Chaoqi Zhang,Yabo Zhou,Dianheng Wang,Li Zhou,Zhenfeng Wang,Nan Sun,Xin Wang,Huafeng Zhang,Ke Tang,Jingwei Ma,Jiadi Lv,Bo HuangPubMed 38099490
- Nature communications 14:70192023Electrocatalytic on-site oxygenation for transplanted cell-based-therapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInkyu Lee,Abhijith Surendran,Samantha Fleury,Ian Gimino,Alexander Curtiss,Cody Fell,Daniel J Shiwarski,Omar Refy,Blaine Rothrock,Seonghan Jo,Tim Schwartzkopff,Abijeet Singh Mehta,Yingqiao Wang,Adam Sipe,Sharon John,Xudong Ji,Georgios Nikiforidis,Adam W Feinberg,Josiah Hester,Douglas J Weber,Omid Veiseh,Jonathan Rivnay,Tzahi Cohen-KarniPubMed 37945597
- Frontiers in cellular and infection microbiology 13:12611892023Multi-function screening of probiotics to improve oral health and evaluating their efficacy in a rat periodontitis model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingqing Nie,Xuchun Wan,Hua Tao,Qianqian Yang,Xueyang Zhao,Haixia Liu,Jun Hu,Yanan Luo,Ting Shu,Ran Geng,Zhijing Gu,Fengkai Fan,Zhi LiuPubMed 38029238
- Nature communications 14:63282023Positive regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomas Venit,Oscar Sapkota,Wael Said Abdrabou,Palanikumar Loganathan,Renu Pasricha,Syed Raza Mahmood,Nadine Hosny El Said,Shimaa Sherif,Sneha Thomas,Salah Abdelrazig,Shady Amin,Davide Bedognetti,Youssef Idaghdour,Mazin Magzoub,Piergiorgio PercipallePubMed 37816864
- Journal of cellular and molecular medicine 27:3189-32012023Hyperbaric oxygen therapy suppresses hypoxia and reoxygenation injury to retinal pigment epithelial cells through activating peroxisome proliferator activator receptor-alpha signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTzong-Bor Sun,Kuo-Feng Huang,Ko-Chi Niu,Cheng-Hsien Lin,Wen-Pin Liu,Chao-Hung Yeh,Shu-Chun Kuo,Ching-Ping ChangPubMed 37731202
- Frontiers in cellular and infection microbiology 13:11134012023Inhibitory effects of vaginal on C growth, hyphal formation, biofilm development, and epithelial cell adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomonori Takano,Hayami Kudo,Shuhei Eguchi,Asami Matsumoto,Kentaro Oka,Yukitaka Yamasaki,Motomichi Takahashi,Takuro Koshikawa,Hiromu Takemura,Yuka Yamagishi,Hiroshige Mikamo,Hiroyuki KunishimaPubMed 37201113
- Archives of pharmacal research 46:323-3382023Regulation of NOX/p38 MAPK/PPARα pathways and miR-155 expression by boswellic acids reduces hepatic injury in experimentally-induced alcoholic liver disease mouse model: novel mechanistic insight.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRania M Salama,Samah S Abbas,Samar F Darwish,Al Aliaa Sallam,Noura F Elmongy,Sara A El WakeelPubMed 36959348
- Journal of medical virology 95:e286762023KSHV hijacks FoxO1 to promote cell proliferation and cellular transformation by antagonizing oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Li,Shou-Jiang GaoPubMed 36929740
Key facts
- Sample types
Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Assay time
1h
- Sensitivity
> 0.04 µM
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Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Sample types
Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Assay time
1h
- Assay Platform
Microplate reader
- Sensitivity
> 0.04 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.
In the hydrogen peroxide assay protocol, horse radish peroxidase (HRP) reacts with a probe and H2O2 to produce a product with color (λmax = 570 nm) and red-fluorescence (Ex/Em=535/587 nm).
The detection limit is 2 pmol per assay (or 40 nM concentration) of H2O2 in the sensitive fluorometric assay.
Hydrogen peroxide assay protocol summary:
- add standards and samples to wells
- add reaction mix and incubate for 10 min
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K265 Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit. K265-200 is the same size as the 200 test size of ab102500.
The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method.
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down's syndrome and immune system diseases.
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3 product images
Vicente, Cl?udia S L et al., PloS one?vol. 10,4 e0123839., Fig 2, doi:10.1371/journal.pone.0123839Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
H2O2 neutralization of of Bursaphelenchus sp. isolates (high virulence B. xylophilus Ka4 and B. xylophilus T4, and low virulence B. xylophilus C14-5 and B. mucronatus Un1) after 24 hours exposition to H2O2.
Error bars represent standard deviation. Different letters above the columns indicate significant differences (p < 0.05) between Bursaphelenchus sp. isolates H2O2 neutralization, according to post-hoc Duncan's test.
Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
H2O2 Fluorimetric Standard Curve
Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
H2O2 Colorimetric Standard Curve
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