Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.
- Cited in > 95 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
Filter by
- In vivo (Athens, Greece) 38:73-812024Ginger Supplementation Attenuated Mitochondrial Fusion and Improved Skeletal Muscle Size in Type 2 Diabetic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCasey R Appell,Nigel C Jiwan,Rui Wang,Chwan-Li Shen,Hui-Ying LukPubMed 38148056
- Scientific reports 14:42792024Kallistatin deficiency exacerbates neuronal damage after cardiac arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayoung Kim,Gil Joon Suh,Woon Yong Kwon,Kyung Su Kim,Yoon Sun Jung,Taegyun Kim,Heesu ParkPubMed 38383562
- The Journal of clinical investigation 133:2023Aryl hydrocarbon receptor sulfenylation promotes glycogenolysis and rescues cancer chemoresistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNannan Zhou,Jie Chen,Zheng Ling,Chaoqi Zhang,Yabo Zhou,Dianheng Wang,Li Zhou,Zhenfeng Wang,Nan Sun,Xin Wang,Huafeng Zhang,Ke Tang,Jingwei Ma,Jiadi Lv,Bo HuangPubMed 38099490
- Nature communications 14:70192023Electrocatalytic on-site oxygenation for transplanted cell-based-therapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInkyu Lee,Abhijith Surendran,Samantha Fleury,Ian Gimino,Alexander Curtiss,Cody Fell,Daniel J Shiwarski,Omar Refy,Blaine Rothrock,Seonghan Jo,Tim Schwartzkopff,Abijeet Singh Mehta,Yingqiao Wang,Adam Sipe,Sharon John,Xudong Ji,Georgios Nikiforidis,Adam W Feinberg,Josiah Hester,Douglas J Weber,Omid Veiseh,Jonathan Rivnay,Tzahi Cohen-KarniPubMed 37945597
- Frontiers in cellular and infection microbiology 13:12611892023Multi-function screening of probiotics to improve oral health and evaluating their efficacy in a rat periodontitis model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingqing Nie,Xuchun Wan,Hua Tao,Qianqian Yang,Xueyang Zhao,Haixia Liu,Jun Hu,Yanan Luo,Ting Shu,Ran Geng,Zhijing Gu,Fengkai Fan,Zhi LiuPubMed 38029238
- Nature communications 14:63282023Positive regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomas Venit,Oscar Sapkota,Wael Said Abdrabou,Palanikumar Loganathan,Renu Pasricha,Syed Raza Mahmood,Nadine Hosny El Said,Shimaa Sherif,Sneha Thomas,Salah Abdelrazig,Shady Amin,Davide Bedognetti,Youssef Idaghdour,Mazin Magzoub,Piergiorgio PercipallePubMed 37816864
- Journal of cellular and molecular medicine 27:3189-32012023Hyperbaric oxygen therapy suppresses hypoxia and reoxygenation injury to retinal pigment epithelial cells through activating peroxisome proliferator activator receptor-alpha signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTzong-Bor Sun,Kuo-Feng Huang,Ko-Chi Niu,Cheng-Hsien Lin,Wen-Pin Liu,Chao-Hung Yeh,Shu-Chun Kuo,Ching-Ping ChangPubMed 37731202
- Frontiers in cellular and infection microbiology 13:11134012023Inhibitory effects of vaginal on C growth, hyphal formation, biofilm development, and epithelial cell adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomonori Takano,Hayami Kudo,Shuhei Eguchi,Asami Matsumoto,Kentaro Oka,Yukitaka Yamasaki,Motomichi Takahashi,Takuro Koshikawa,Hiromu Takemura,Yuka Yamagishi,Hiroshige Mikamo,Hiroyuki KunishimaPubMed 37201113
- Archives of pharmacal research 46:323-3382023Regulation of NOX/p38 MAPK/PPARα pathways and miR-155 expression by boswellic acids reduces hepatic injury in experimentally-induced alcoholic liver disease mouse model: novel mechanistic insight.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRania M Salama,Samah S Abbas,Samar F Darwish,Al Aliaa Sallam,Noura F Elmongy,Sara A El WakeelPubMed 36959348
- Journal of medical virology 95:e286762023KSHV hijacks FoxO1 to promote cell proliferation and cellular transformation by antagonizing oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Li,Shou-Jiang GaoPubMed 36929740
Key facts
- Sample types
- Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
- Quantitative
- Assay time
- 1h
- Sensitivity
- > 0.04 µM
Associated Products
Select an associated product type
2 products for Alternative Product
What's included?
Recommended products
Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.
- Cited in > 95 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Sample types
- Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
- Quantitative
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- > 0.04 µM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.
The detection limit is 2 pmol per assay (or 40 nM concentration) of H2O2 with the more sensitive fluorometric readout.
The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method.
Hydrogen peroxide assay principle
In the hydrogen peroxide assay protocol, horse radish peroxidase (HRP) reacts with a probe and H2O2 to produce a product with color (λmax = 570 nm) and red-fluorescence (Ex/Em=535/587 nm).
Hydrogen peroxide assay protocol summary:
- Add standards and samples to wells
- Add reaction mix and incubate for 10 min
- Analyze with microplate reader
How other researchers are using
Hydrogen Peroxide Assay Kit has been used in a variety of sample type including:
- Mouse breast tumor cells 1
- Mouse embryonic fibroblasts 2
- Vaginal strains 3
References:
1- Zhou N et al. 2023; 2- Venit T et al. 2023
3- Takano T et al. 2023
Related and recommended products
See other alternative kits to quantify Hydrogen Peroxide:
- Hydrogen Peroxide Assay Kit (Cell-based) Hydrogen Peroxide Assay Kit (Cell-based) ab138874
- Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared) Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared) ab138886
Other notes
This product was previously called K265 Biovision Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down's syndrome and immune system diseases.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
H2O2 Fluorimetric Standard Curve
Vicente, Claudia S L et al., PloS one: vol. 10,4 e0123839., Fig 2, doi:10.1371/journal.pone.0123839Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
H2O2 neutralization of of Bursaphelenchus sp. isolates (high virulence B. xylophilus Ka4 and B. xylophilus T4, and low virulence B. xylophilus C14-5 and B. mucronatus Un1) after 24 hours exposition to H2O2.
Error bars represent standard deviation. Different letters above the columns indicate significant differences (p < 0.05) between Bursaphelenchus sp. isolates H2O2 neutralization, according to post-hoc Duncan's test.
Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
H2O2 Colorimetric Standard Curve
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com