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Hydrogen Peroxide Assay Kit (Cell-based) (ab138874) uses our AbGreen Indicator to quantify hydrogen peroxide in live cells.


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Functional Studies - Hydrogen Peroxide Assay Kit (Cell-based) (AB138874), expandable thumbnail

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Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Reactive species

Mammals

Assay time

30m

Sensitivity

= 3 µM

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What's included?

200 Test
Components
AbGreen Indicator
1 x 1 Vial
Assay Buffer
1 x 20 mL
DMSO
1 x 200 µL
Hydrogen Peroxide Solution
1 x 200 µL

Recommended products

Hydrogen Peroxide Assay Kit (Cell-based) (ab138874) uses our AbGreen Indicator to quantify hydrogen peroxide in live cells.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Reactive species

Mammals

Assay time

30m

Assay Platform

Fluorescence microscope

Sensitivity

= 3 µM

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Hydrogen Peroxide Assay Kit (Cell-based) (ab138874) uses our AbGreen Indicator to quantify hydrogen peroxide in live cells. The indicator is cell-permeable, and generates green fluorescence when it reacts with hydrogen peroxide.

The assay is in a "mix and read" assay format that is compatible with HTS liquid handling instruments and a 96-well or 384-well plate format. It provides a sensitive, one-step fluorometric assay to detect as little as 0.3 nanomoles of H2O2 in a 100 μL assay volume (3 μM). Results can be read by either a fluorescence microplate reader at Ex/Em = 490/520 nm for H2O2 detection in solution or a fluorescence microscopy for live cell H2O2 imaging.

Hydrogen peroxide assay protocol summary:
- add samples and standards to wells
- add green indicator solution
- analyze with microplate reader for 15-60 min.

Alternatively, for live cell assay:
- add green indicator solution to cells
- incubate for 5-60 min whilst analyzing with microplate reader or fluorescence microscope

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in many biological events that are linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. The measurement of this reactive species is helpful for determining how oxidative stress modulates various intracellular pathways.

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Functional Studies - Hydrogen Peroxide Assay Kit (Cell-based) (ab138874), expandable thumbnail

    Functional Studies - Hydrogen Peroxide Assay Kit (Cell-based) (ab138874)

    Fluorescence images of intracellular hydrogen peroxide in HeLa cells using Hydrogen Peroxide Assay Kit (Cell-based) (ab138874). HeLa cells were treated with (left) or without (right) 100 μM hydrogen peroxide at 37°C for 90 minutes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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