Skip to main content

Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared) (ab138886) uses our unique AbIR Peroxidase Indicator substrate to quantify hydrogen peroxide in solutions and cell extracts.

Be the first to review this product! Submit a review

Images

Functional Studies - Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared) (AB138886), expandable thumbnail

Publications

Key facts

Detection method

Fluorescent

Sample types

Cell culture supernatant, Adherent cells

Assay type

Enzyme activity

Reactive species

Mammals

Sensitivity

= 30 nM

Associated Products

Select an associated product type

2 products for Alternative Product

What's included?

500 Test
Components
Assay Buffer
1 x 100 mL
DMSO
1 x 0.5 mL
Horseradish Peroxidase
1 x 20 Unit
Hydrogen Peroxide Solution
1 x 200 µL
IR Peroxidase Indicator
1 x 1 Vial

Recommended products

Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared) (ab138886) uses our unique AbIR Peroxidase Indicator substrate to quantify hydrogen peroxide in solutions and cell extracts.

Key facts

Detection method

Fluorescent

Sample types

Cell culture supernatant, Adherent cells

Assay type

Enzyme activity

Reactive species

Mammals

Assay Platform

Microplate reader

Sensitivity

= 30 nM

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared) (ab138886) uses our unique AbIR Peroxidase Indicator substrate to quantify hydrogen peroxide in solutions and cell extracts. AbIR Peroxidase Indicator generates the fluorescence that is pH-independent from pH 4 to 10. It is a superior alternative to ADHP for the detections that require low pH where ADHP has reduced fluorescence. In addition, AbIR Peroxidase Indicator generates a product that has maximum absorption at 647 nm with maximum emission at 670 nm. This near infrared fluorescence minimizes the assay background that is often caused by the autofluorescence of biological samples. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions.

This is an optimized "mix and read" assay that is compatible with HTS liquid handling instruments. It provides a sensitive, one-step fluorometric assay to detect as little as 3 pmol H2O2 /100 μL assay volume (30 nM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = ~640/680 nm or an absorbance microplate reader at ~650 nm. Due to its long emission wavelength, this kit has low interference from biological samples.

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in a number of biological events that have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. Perhaps the most intriguing aspect of H2O2 biology is the recent report that antibodies have the capacity to convert molecular oxygen into hydrogen peroxide to contribute to the normal recognition and destruction processes of the immune system. Measurement of this reactive species will help to determine how oxidative stress modulates a variety of intracellular pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared) (ab138886), expandable thumbnail

    Functional Studies - Hydrogen Peroxide Assay Kit (Fluorometric - Near Infrared) (ab138886)

    Sample Standard Curve for Hydrogen Peroxidase. Hydrogen Peroxidase dose response was measured on a 96-well black plate with Hydrogen Peroxidase Assay Kit (Fluorometric-Near Infrared), using a fluorescence microplate reader (Molecular Devices). As low as 0.03 µM H2O2 can be detected with 1 minute incubation time (n=3). The insert shows the low levels of Hydrogen Peroxidase detection.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com