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AB117131

Hydroxymethylated DNA Quantification Kit (Fluorometric)

3

(1 Review)

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(2 Publications)

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).
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Functional Studies - Hydroxymethylated DNA Quantification Kit (Fluorometric) (AB117131)
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Supplier Data

Functional Studies - Hydroxymethylated DNA Quantification Kit (Fluorometric) (AB117131)

Demonstration of high sensitivity and specificity of 5-hydroxymethylcytosine detection achieved using ab117131. Synthetic unmethylated DNA (contains only cytosine), methylated DNA (contains only 5-methylcytosine), and hydroxymethylated DNA standard (contains only 5-hydroxymethylcytosine) were added into the assay wells at different concentrations and then measured with ab117131.

Key facts

Detection method

Fluorescent

Sample types

Saliva, Urine, Plasma, Tissue, Suspension cells, Adherent cells

Results type

Quantitative

Sensitivity

>= 10 pg/well

Assay time

3h 20m

Assay Platform

Microplate reader

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AB233487

Global DNA Hydroxymethylation Assay Kit (5hmc, Colorimetric)

Functional Studies - Global DNA Hydroxymethylation Assay Kit (5hmc, Colorimetric) (AB233487)

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Product details

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.

Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.

It can be paired to use with EpiSeeker methylated DNA Quantification Kit (Fluorometric) (ab117129), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 16:e1008823 PubMed32453789

2020

Glucose transporter 10 modulates adipogenesis via an ascorbic acid-mediated pathway to protect mice against diet-induced metabolic dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chung-Lin Jiang,Wei-Ping Jen,Chang-Yu Tsao,Li-Ching Chang,Chien-Hsiun Chen,Yi-Ching Lee

Endocrine connections 6:708-725 PubMed28993426

2017

Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jairo Arturo Pinzón-Cortés,Angelina Perna-Chaux,Nicolás Steven Rojas-Villamizar,Angélica Díaz-Basabe,Diana Carolina Polanía-Villanueva,María Fernanda Jácome,Carlos Olimpo Mendivil,Helena Groot,Valeriano López-Segura
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Product promise

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