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AB219929

Hypochlorite Assay Kit (Colorimetric)

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(1 Publication)

Hypochlorite Detection Kit (Colorimetric) (ab219929) provides a sensitive assay for measuring hypochlorite (HClO, hypochlorous acid) with high specificity in cell and tissue lysates and biological fluids.
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Functional Studies - Hypochlorite Assay Kit (Colorimetric) (AB219929)
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Supplier Data

Functional Studies - Hypochlorite Assay Kit (Colorimetric) (AB219929)

Typical Hypochlorite standard calibration curve. Hypochlorite was measured with in a 96 well clear bottom plate. As low as 0.001% (10 ppm) sodium hypochlorite (NaClO) was detected with 3-5 minutes incubation.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Sensitivity

= 0.001 %

Assay Platform

Microplate reader

Product details

Hypochlorite Detection Kit (Colorimetric) (ab219929) provides a sensitive assay for measuring hypochlorite (HClO, hypochlorous acid) with high specificity in cell and tissue lysates and biological fluids. The hypochlorite sensor used in the assay selectively reacts with hypochlorite (hypochlorous) to generate a red color product which can be measured with an absorbance microplate reader at OD 555 nm.

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 8: PubMed31947581

2020

Development and Optimization of a GMP-Compliant Manufacturing Process for a Personalized Tumor Lysate Dendritic Cell Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Boudousquié,Valérie Boand,Emilie Lingre,Laeticia Dutoit,Klara Balint,Maxime Danilo,Alexandre Harari,Philippe O Gannon,Lana E Kandalaft
View all publications
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