Hypochlorite Assay Kit (Fluorometric)

Product details

Hypochlorite Detection Kit (Fluorimetric) (ab219930) provides a sensitive fluorescence-based assay for measuring hypochlorite (HClO, hypochlorous acid) with high specificity in cell and tissue lysates and biological fluids. Upon selective reaction with hypochlorite (hypochlorous), the weakly fluorescent Hypochlorite Sensor used in the assay selectively reacts with hypochlorite (hypochlorous) generates a strongly fluorescent product with more than 100-fold fluorescence enhancement. The fluorescence signal can be measured by a fluorescence microplate reader at Ex/Em = 540/590 nm. The assay can detect as low as 3 μM hypochlorite was detected in a 100 μL reaction volume.

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance.