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AB325583

In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation)

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ab325583 In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation) kit provides a stringent, anchorage-independent model for chemosensitivity testing and potential anti-cancer drug screening. Each kit provides sufficient quantities to perform 96 tests in a microtiter plate.
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Functional Studies - In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation) (AB325583)
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Supplier Data

Functional Studies - In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation) (AB325583)

Inhibition of HeLa Cell Anchorage-Independent Growth by Taxol. HeLa cells were cultured for 7 days in the absence (left) or presence (right) of 1 nM Taxol according to the assay protocol.

This figure demonstrates typical results with ab325583 In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation). One should use the data below for reference only. This data should not be used to interpret actual results.

Functional Studies - In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation) (AB325583)
  • FuncS

Supplier Data

Functional Studies - In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation) (AB325583)

Inhibition of Hela Cell Transformation by 5-Fluorouracil. HeLa cells were seeded at 5000 cells/well and cultured 7 days at various 5-FU concentrations. Cell transformation was determined according to the assay protocol. IC50 value of 5-Fluorouracil on HeLa cell anchorage-independent growth was determined to be around 1 μM.

This figure demonstrates typical results with ab325583 In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation). One should use the data below for reference only. This data should not be used to interpret actual results.

Functional Studies - In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation) (AB325583)
  • FuncS

Supplier Data

Functional Studies - In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation) (AB325583)

HeLa Cell Dose Curve. Cervical carcinoma HeLa cells were resuspended at 6 x 106 cells/mL and titrated 1 : 2 in culture medium, followed by addition of Cell Dose Curve Solution, Matrix Solubilization Solution, MTT Solution, and Detergent Solution (as described in the Cell Dose Section). Results are shown by cell concentration or by actual cell number in MTT Detection.

This figure demonstrates typical results with ab325583 In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation). One should use the data below for reference only. This data should not be used to interpret actual results.

Key facts

Detection method

Colorimetric

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cellular Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Tumor sensitivity assays are intended to help predict the sensitivity of various tumors to chemotherapeutic agents, with the intent of identifying the most effective treatment with the fewest side effects. With this information, physicians can devise tailor made chemotherapy regiments and eliminate ineffective drugs, sparing patients of unnecessary toxicity. Ideally, an in vitro tumor sensitivity assay must be reliable, sensitive, and resemble the 3-D, in vivo environment (such as culturing in collagen gel or soft agar).

Traditionally, the soft agar colony formation assay is a common method to monitor anchorage-independent growth, which measures proliferation in a semi-solid culture media after 3-4 weeks by manual counting of colonies.

ab325583 In Vitro Tumor Sensitivity Assay (Soft Agar Colony Formation) kit does not involve subjective manual counting of colonies or require a 3–4-week incubation period. Instead, cells are incubated only 6-8 days in a proprietary semi-solid agar media before being solubilized, transferred and detected by the provided MTT Solution in a microtiter plate reader.

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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab325583-96Tests", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"MTT Solution", "number":"AB325583-CMP05", "productcode":"" }, { "size":"1 x 4 mL", "name":"Matrix Diluent", "number":"AB325583-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"10X Agar Matrix Solution", "number":"AB325583-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"1X Matrix Solubilization Buffer", "number":"AB325583-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Detergent Solution", "number":"AB325583-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"5X DMEM Medium", "number":"AB325583-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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