Indican Assay Kit

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Indican also known as 3-indoxyl sulfate is a byproduct of the metabolism of tryptophan. It commonly occurs in the liver where it is derived from the bacterial breakdown of tryptophan in the gut producing indole that reaches the liver through the portal circulation. Within the liver indole undergoes a transformation into indican which contains a molecular mass of approximately 213.2 g/mol. This compound is frequently excreted in the urine and can be quantified through urinary indican tests or kits providing a measure of gut bacterial activity and liver function.

Biological function summary

This metabolite participates in liver detoxification processes and is often associated with the excretory function of the kidneys. As a conjugated form of indole indican correlates with the levels of tryptophan degradation and the body's ability to process indoles efficiently. Although not a part of a large protein complex indican reflects the gut's microbial metabolic activity and influences the transport function in renal processing. In the circulatory system its concentration serves as an indicator of intestinal bacterial overgrowth and familiarizes researchers with absorption and nutritional metabolism insights.

Pathways

The metabolism and excretion of indican indicate involvement in the indole metabolic pathway and link to oxidative metabolism related to aromatic amino acids like tryptophan. This connection highlights its relationship with liver detoxification pathways and involves transport proteins like OAT1 (Organic Anion Transporter 1) and OAT3. Both OAT1 and OAT3 play roles in transporting indican from the blood into the urine emphasizing the compound's role in renal clearance pathways and its importance in maintaining homeostasis in systemic circulation.

Associated diseases and disorders

Elevated indican levels relate to intestinal conditions like bacterial overgrowth syndrome and hepatic dysfunction including liver cirrhosis. These conditions connect to proteins involved in metabolism and detoxification processes such as cytochrome P450 enzymes which drive the liver's capacity to handle byproducts from microbial activity in the gut. Additionally the role of OAT1 and OAT3 in renal excretion of indican provides insight into balance disruptions associated with chronic kidney disease. Understanding the interactions between indican levels and these conditions helps advance diagnostic tools and offers therapeutic insights into managing digestive and renal health.