Indole assay kit (272536) is based on a modified version of Ehlrich's and Kovac's reagents, which reacts with indole to produce a colored compound at 565 nm.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Other biological fluids
- Assay type
- Quantitative
- Range
- 3 - 100 µM
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
Recommended products
Indole assay kit (272536) is based on a modified version of Ehlrich's and Kovac's reagents, which reacts with indole to produce a colored compound at 565 nm.
Key facts
- Detection method
- Colorimetric
- Sample types
- Other biological fluids
- Assay type
- Quantitative
- Range
- 3 - 100 µM
- Assay Platform
- Microplate
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Indole assay kit (272536) is based on a modified version of Ehlrich's and Kovac's reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample.
Fast and sensitive: Use of 100 μL sample. Linear detection range from 3 to 100 μM indole in 96-well plate assay.
Convenient: The procedure involves adding a single working reagent, and reading the absorbance immediately.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Indole an organic compound with molecular mass of 117.15 g/mol plays a significant role in various biological systems. It can be found across a wide range of organisms particularly in both human and bacterial systems. In bacterial settings such as in Escherichia coli (E. coli) it serves as a product of tryptophan metabolism. It is known by alternative names including 'indole reagent' and 'Kovac's indole reagent' the latter of which is essential in the indole test procedure. The presence of indole in bacterial cultures can be detected using the indole assay which aids in identifying bacteria that can produce indole.
- Biological function summary
Indole influences microbial ecology and cell communication in a variety of ways. It functions as an intercellular signal molecule that impacts growth development and biofilm formation in bacteria. It is not part of a complex but interacts with other signaling molecules. In E. coli the indole test is often employed to determine the ability of bacteria to degrade the amino acid tryptophan to indole therefore indicating certain metabolic capabilities and pathways.
- Pathways
Indole is central to the tryptophan metabolic pathway. It works alongside proteins such as tryptophanase which catalyzes the conversion of tryptophan to indole. Moreover indole interacts with the so-called indole-3-acetic acid (IAA) pathway which involves auxin a plant hormone essential for growth and development. These pathways highlight indole’s multifunctional role bridging microbial and plant systems through shared biochemical routes.
- Associated diseases and disorders
Indole has been associated with cancer and gastrointestinal disorders. In cancer biology aberrant metabolism of tryptophan leading to altered indole levels can affect tumor growth and immune response. Indole is connected to proteins involved in these processes such as aryl hydrocarbon receptor (AhR) which mediates indole-related signaling paths. In gastrointestinal disorders indole and its derivatives can impact gut microbiota composition interacting with proteins and pathways that influence gut health and inflammation.
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2 product images
Functional Studies - Indole Assay Kit (ab272536)
Indole Assay Kit Standard Curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Functional Studies - Indole Assay Kit (ab272536)
E.coli Growth and Indole Concentration.
E.coli cells inoculated into 5 mM Tryptophan medium. Medium samples taken every two hours.
Data provided for demonstration purposes only.
Downloads
Product protocols
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