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Inorganic Polyphosphate Assay Kit (Fluorometric) (ab284528) (K2025) is a simple assay to quantify the amount of Poly P in various biological samples.

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Images

Functional Studies - Inorganic Polyphosphate Assay Kit (Fluorometric) (AB284528), expandable thumbnail
  • Functional Studies - Inorganic Polyphosphate Assay Kit (Fluorometric) (AB284528), expandable thumbnail

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell Lysate

Assay type

Cell-based (quantitative)

Reactivity data

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

What's included?

100 Test
Components
Buffer III
1 x 30 mL
DNase
1 x 400 µL
Polyphosphate Assay Buffer
1 x 100 mL
Polyphosphate Dye
1 x 1 Vial
Polyphosphate Standard
1 x 1 Vial
Proteinase K
1 x 200 µL
RNAse Positive Control
1 x 400 µL

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Inorganic Polyphosphate Assay Kit (Fluorometric) (ab284528) (K2025) is a simple assay to quantify the amount of Poly P in various biological samples.

Key facts

Detection method

Fluorescent

Sample types

Tissue Lysate, Cell Lysate

Assay type

Cell-based (quantitative)

Assay Platform

Microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Inorganic Polyphosphate Assay Kit (Fluorometric) (ab284528) (K2025) is a simple assay to quantify the amount of Poly P in various biological samples. The assay uses a fluorescent dye that forms a complex with Poly P present in the samples and the fluorsecent complex is measured at Ex/Em = 415/550 nm.

The fluorescent signal is proportional to the Poly P concentration in the samples.

This kit provides a rapid, sensitive and a convenient tool for detecting Poly P. It can detect as low as 50 pmole under the assay conditions.

This product is manufactured by BioVision, an Abcam company and was previously called K2025 Inorganic Polyphosphate Assay Kit (Fluorometric). K2025-100 is the same size as the 100 test size of ab284528.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Inorganic Polyphosphate Assay Kit (Fluorometric) (ab284528), expandable thumbnail

    Functional Studies - Inorganic Polyphosphate Assay Kit (Fluorometric) (ab284528)

    Inorganic Polyphosphate Assay.

    Poly P Standard Curve.

  • Functional Studies - Inorganic Polyphosphate Assay Kit (Fluorometric) (ab284528), expandable thumbnail

    Functional Studies - Inorganic Polyphosphate Assay Kit (Fluorometric) (ab284528)

    Inorganic Polyphosphate Assay.

    Poly P concentration in rat brain tissue and E. coli cells after osmotic stress assayed according to the kit protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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