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AB126286

Inosine Assay Kit (Fluorometric)

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(7 Publications)

Inosine Assay Kit ab126286 is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Inosine Assay Kit (Fluorometric) (AB126286)
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Supplier Data

Functional Studies - Inosine Assay Kit (Fluorometric) (AB126286)

Inosine standard curve generated using this kit protocol.

Key facts

Detection method

Fluorescent

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

= 0.1 nmol/well

Assay time

40m

Assay Platform

Microplate reader

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Product details

Inosine Assay Kit (Fluorometric) (ab126286) is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.

Other Notes
This product was previously called K712 Inosine Fluorometric Assay Kit and Inosine Quantification Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab126286-100Test", "assetComponentDetails": [ { "size":"1 x 0.4 mL", "name":"PicoProbe I", "number":"AB126286-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Substrate Mix I", "number":"AB126286-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix J", "number":"AB126286-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix E", "number":"AB126286-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 50", "number":"AB126286-CMP02", "productcode":"" }, { "size":"1 x 50 µL", "name":"Inosine Standard", "number":"AB126286-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inosine a nucleoside plays a mechanical role in transfer RNA (tRNA) modification and purine metabolism. It commonly gets referred to as hypoxanthine riboside. Inosine has a molecular weight of 268.24 g/mol making it a relatively small molecule. It is broadly expressed in various tissues including muscle and nerve tissues due to its involvement in nucleotide synthesis and cellular energy metabolism.
Biological function summary

Inosine participates in diverse cellular processes beyond its role in nucleoside modification. Inosine forms part of a complex network where it impacts RNA editing and stability influencing protein translation. Its conversion to hypoxanthine via deamination is significant for the purine salvage pathway allowing cells to recycle purines efficiently. This activity underlines the importance of inosine in maintaining nucleotide balance and supporting cellular functions particularly in rapidly proliferating or energy-demanding cells.

Pathways

Inosine's involvement in the purine salvage and nucleotide metabolism pathways highlights its pivotal role in cellular homeostasis. The purine salvage pathway is essential for the recycling of nucleotides which inosine facilitates by converting to hypoxanthine and then to xanthine. Inosine interacts with proteins like xanthine oxidase part of the same metabolic pathway thereby ensuring efficient nucleotide utilization and preventing wasteful de novo synthesis.

Some studies have linked inosine to neurological disorders like multiple sclerosis and amyotrophic lateral sclerosis. Inosine shows potential in neuroprotection by increasing adenosine levels and promoting axonal regeneration. It interacts with adenosine deaminase which influences immune responses. Furthermore imbalances in inosine metabolism can exacerbate conditions related to oxidative stress and energy deficiency suggesting a therapeutic angle for inosine in specific neurological diseases.
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Product protocols

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Target data

See full target information Inosine
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:416 PubMed38988443

2024

Purine metabolite inosine induced by transforming growth factor‑β promotes epithelial‑mesenchymal transition in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weimin Hu,Ling Cheng,Jian Zhao,Yonghui Wu,Ying Lin,Jun Yin

Cell reports 42:112078 PubMed36735535

2023

Loss of C3a and C5a receptors promotes adipocyte browning and attenuates diet-induced obesity via activating inosine/A2aR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Ran Kong,Xiao-Hui Chen,Qing Sun,Kai-Yuan Zhang,Lian Xu,Liliqiang Ding,Yan-Ping Zhou,Ze-Bei Zhang,Jing-Rong Lin,Ping-Jin Gao

Journal of inherited metabolic disease 46:421-435 PubMed36371683

2022

Cellular and computational models reveal environmental and metabolic interactions in MMUT-type methylmalonic aciduria.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Ramon,Florian Traversi,Céline Bürer,D Sean Froese,Jörg Stelling

Cell reports 37:109897 PubMed34706243

2021

Adenosine deaminase 2 produced by infiltrative monocytes promotes liver fibrosis in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shilpa Tiwari-Heckler,Eric U Yee,Yusuf Yalcin,Jiwoon Park,Duc-Huy T Nguyen,Wenda Gao,Eva Csizmadia,Nezam Afdhal,Kenneth J Mukamal,Simon C Robson,Michelle Lai,Robert E Schwartz,Z Gordon Jiang

Cardiovascular research 118:2847-2858 PubMed34668514

2021

Ogfod1 deletion increases cardiac beta-alanine levels and protects mice against ischaemia- reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Harris,Junhui Sun,Karen Keeran,Angel Aponte,Komudi Singh,Danielle Springer,Marjan Gucek,Mehdi Pirooznia,Matthew E Cockman,Elizabeth Murphy,Leslie M Kennedy

Cell host & microbe 29:1351-1365.e11 PubMed34403684

2021

Gut microbiome ADP-ribosyltransferases are widespread phage-encoded fitness factors.

Applications

Unspecified application

Species

Unspecified reactive species

Eric M Brown,Hugo Arellano-Santoyo,Emily R Temple,Zachary A Costliow,Matthieu Pichaud,A Brantley Hall,Kai Liu,Michael A Durney,Xiebin Gu,Damian R Plichta,Clary A Clish,Jeffrey A Porter,Hera Vlamakis,Ramnik J Xavier

PloS one 10:e0123509 PubMed25856396

2015

Opposing activity changes in AMP deaminase and AMP-activated protein kinase in the hibernating ground squirrel.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Lanaspa,L Elaine Epperson,Nanxing Li,Christina Cicerchi,Gabriela E Garcia,Carlos A Roncal-Jimenez,Jessica Trostel,Swati Jain,Colin T Mant,Christopher J Rivard,Takuji Ishimoto,Michiko Shimada,Laura Gabriela Sanchez-Lozada,Takahiko Nakagawa,Alkesh Jani,Peter Stenvinkel,Sandra L Martin,Richard J Johnson
View all publications
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