Inosine Quantification Assay Kit ab126286 is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cell reports 42:1120782023Loss of C3a and C5a receptors promotes adipocyte browning and attenuates diet-induced obesity via activating inosine/A2aR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing-Ran Kong,Xiao-Hui Chen,Qing Sun,Kai-Yuan Zhang,Lian Xu,Liliqiang Ding,Yan-Ping Zhou,Ze-Bei Zhang,Jing-Rong Lin,Ping-Jin GaoPubMed 36735535
- Journal of inherited metabolic disease 46:421-4352022Cellular and computational models reveal environmental and metabolic interactions in MMUT-type methylmalonic aciduria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte Ramon,Florian Traversi,Céline Bürer,D Sean Froese,Jörg StellingPubMed 36371683
- Cell reports 37:1098972021Adenosine deaminase 2 produced by infiltrative monocytes promotes liver fibrosis in nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShilpa Tiwari-Heckler,Eric U Yee,Yusuf Yalcin,Jiwoon Park,Duc-Huy T Nguyen,Wenda Gao,Eva Csizmadia,Nezam Afdhal,Kenneth J Mukamal,Simon C Robson,Michelle Lai,Robert E Schwartz,Z Gordon JiangPubMed 34706243
- Cardiovascular research 118:2847-28582021Ogfod1 deletion increases cardiac beta-alanine levels and protects mice against ischaemia- reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Harris,Junhui Sun,Karen Keeran,Angel Aponte,Komudi Singh,Danielle Springer,Marjan Gucek,Mehdi Pirooznia,Matthew E Cockman,Elizabeth Murphy,Leslie M KennedyPubMed 34668514
- Cell host & microbe 29:1351-1365.e112021Gut microbiome ADP-ribosyltransferases are widespread phage-encoded fitness factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric M Brown,Hugo Arellano-Santoyo,Emily R Temple,Zachary A Costliow,Matthieu Pichaud,A Brantley Hall,Kai Liu,Michael A Durney,Xiebin Gu,Damian R Plichta,Clary A Clish,Jeffrey A Porter,Hera Vlamakis,Ramnik J XavierPubMed 34403684
- PloS one 10:e01235092015Opposing activity changes in AMP deaminase and AMP-activated protein kinase in the hibernating ground squirrel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiguel A Lanaspa,L Elaine Epperson,Nanxing Li,Christina Cicerchi,Gabriela E Garcia,Carlos A Roncal-Jimenez,Jessica Trostel,Swati Jain,Colin T Mant,Christopher J Rivard,Takuji Ishimoto,Michiko Shimada,Laura Gabriela Sanchez-Lozada,Takahiko Nakagawa,Alkesh Jani,Peter Stenvinkel,Sandra L Martin,Richard J JohnsonPubMed 25856396
Key facts
- Detection method
Fluorescent
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Reactive species
Mammals
- Assay time
40m
- Sensitivity
= 0.1 nmol/well
Target data
What's included?
Recommended products
Inosine Quantification Assay Kit ab126286 is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Reactive species
Mammals
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
= 0.1 nmol/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Inosine Quantification Assay Kit (Fluorometric) (ab126286) is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.
This product is manufactured by BioVision, an Abcam company and was previously called K712 Inosine Fluorometric Assay Kit. K712-100 is the same size as the 100 test size of ab126286.
Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Inosine a nucleoside plays a mechanical role in transfer RNA (tRNA) modification and purine metabolism. It commonly gets referred to as hypoxanthine riboside. Inosine has a molecular weight of 268.24 g/mol making it a relatively small molecule. It is broadly expressed in various tissues including muscle and nerve tissues due to its involvement in nucleotide synthesis and cellular energy metabolism.
- Biological function summary
Inosine participates in diverse cellular processes beyond its role in nucleoside modification. Inosine forms part of a complex network where it impacts RNA editing and stability influencing protein translation. Its conversion to hypoxanthine via deamination is significant for the purine salvage pathway allowing cells to recycle purines efficiently. This activity underlines the importance of inosine in maintaining nucleotide balance and supporting cellular functions particularly in rapidly proliferating or energy-demanding cells.
- Pathways
Inosine's involvement in the purine salvage and nucleotide metabolism pathways highlights its pivotal role in cellular homeostasis. The purine salvage pathway is essential for the recycling of nucleotides which inosine facilitates by converting to hypoxanthine and then to xanthine. Inosine interacts with proteins like xanthine oxidase part of the same metabolic pathway thereby ensuring efficient nucleotide utilization and preventing wasteful de novo synthesis.
- Associated diseases and disorders
Some studies have linked inosine to neurological disorders like multiple sclerosis and amyotrophic lateral sclerosis. Inosine shows potential in neuroprotection by increasing adenosine levels and promoting axonal regeneration. It interacts with adenosine deaminase which influences immune responses. Furthermore imbalances in inosine metabolism can exacerbate conditions related to oxidative stress and energy deficiency suggesting a therapeutic angle for inosine in specific neurological diseases.
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Functional Studies - Inosine Quantification Assay Kit (Fluorometric) (ab126286)
Inosine standard curve generated using this kit protocol.
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