Intracellular Oxygen Concentration Assay (ab197245) is an easy mix and measure 96 well fluorescence plate reader based approach for the analysis of intracellular oxygen concentration at the cell monolayer.
Images
Publications
Filter by
- Molecules (Basel, Switzerland) 28:2023Salvianolic Acid B Inhibits Ferroptosis and Apoptosis during Myocardial Ischemia/Reperfusion Injury via Decreasing the Ubiquitin-Proteasome Degradation of GPX4 and the ROS-JNK/MAPK Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojin Xu,Chenhan Mao,Chengbo Zhang,Meng Zhang,Jianbin Gong,Xindong WangPubMed 37241859
- Cancer research 82:2417-24302022Hypoxia-Reoxygenation Couples 3βHSD1 Enzyme and Cofactor Upregulation to Facilitate Androgen Biosynthesis and Hormone Therapy Resistance in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Qin,Yoon-Mi Chung,Michael Berk,Bryan Naelitz,Ziqi Zhu,Eric Klein,Abhishek A Chakraborty,Nima SharifiPubMed 35536859
- MicrobiologyOpen 8:e006752018Wolbachia pipientis grows in Saccharomyces cerevisiae evoking early death of the host and deregulation of mitochondrial metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Uribe-Alvarez,Natalia Chiquete-Félix,Lilia Morales-García,Arlette Bohórquez-Hernández,Norma Laura Delgado-Buenrostro,Luis Vaca,Antonio Peña,Salvador Uribe-CarvajalPubMed 29897678
- Mediators of inflammation 2018:73739212018Obstructive Sleep Apnea Monocytes Exhibit High Levels of Vascular Endothelial Growth Factor Secretion, Augmenting Tumor Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolina Cubillos-Zapata et. al.PubMed 29997451
- The European respiratory journal 49:2017Monocytes inhibit NK activity TGF-β in patients with obstructive sleep apnoea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnrique Hernández-Jiménez et. al.PubMed 28619958
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells, Suspension cells
- Assay type
- Quantitative
- Reactive species
- Human
- Assay time
- 1h 30m
What's included?
Recommended products
Intracellular Oxygen Concentration Assay (ab197245) is an easy mix and measure 96 well fluorescence plate reader based approach for the analysis of intracellular oxygen concentration at the cell monolayer.
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells, Suspension cells
- Assay type
- Quantitative
- Reactive species
- Human
- Assay time
- 1h 30m
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Intracellular Oxygen Concentration Assay (ab197245) is an easy mix and measure 96 well fluorescence plate reader based approach for the analysis of intracellular oxygen concentration at the cell monolayer. The assay is based on the ability of oxygen to quench the excited state of the oxygen-sensitive probe. The probe is taken up via nonspecific energy dependent endocytosis and, after washing, the cells are monitored on a fluorescence plate reader (dual-read TR-F required for full oxygen quantitation). The probe phosphorescence is quenched by intracellular oxygen in a non-chemical, reversible manner allowing the measurement of average intracellular O2 levels and facilitating real-time monitoring of relative changes in cellular oxygen consumption. The probe signal increases with a reduction in intracellular oxygen and deceases with an increase in intracellular oxygen. The probe is excitable at 360-400 or 535 nm and emits at 630-680 nm. Optimal filter combinations are Ex/Em = 340/642 nm.
The flexible plate reader format, allows multiparametric or multiplex combination with a range of other reagents and it is suitable for HTP automation.
Learn more about the full range of . Or review the full for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Cellular Activity - Intracellular Oxygen Concentration Assay (ab197245)
Excitation and emission spectra of O2 probe.
Excitation and Emission spectra of Intracellular O2 probe, showing normalized excitation (Ex 360-400nm; Peak 380nm) and emission (Em 630-670nm; Peak 650nm).
Cellular Activity - Intracellular Oxygen Concentration Assay (ab197245)
Cellular Activity - Intracellular Oxygen Concentration Assay (ab197245)
HEK293T cell oxygenation. HEK293T cells were cultured in 2D and measured at ambient oxygen. Intracellular O2 levels were ~ 14%. Reducing instrument O2 to 6% caused cellular oxygenation to drop to ~2%. Assay performed using a CLARIOstart equipped with an ACU module (BMG Labtech).
Cellular Activity - Intracellular Oxygen Concentration Assay (ab197245)
Sample callibration data.
Sample Calibration Data. Intra O2 probe Lifetime profiles measured at decreasing [O2] with parallel glucose oxidase treatment to achieve 0% O2.
Cellular Activation - Intracellular Oxygen Concentration Assay (ab197245)
Relationship between probe lifetime (τ) and applied [O2].
Relationship between probe lifetime (τ) and applied [O2]. Applying a first order exponential fit generates a calibration function of O2% = A1 x Exp(-τ / t1). Example: O2% = 659.3 x Exp(-τ / 8.475).
Cellular Activation - Intracellular Oxygen Concentration Assay (ab197245)
Measuring the impact of cell metabolism on iPS-derived cardiomyocyte oxygenation. During measurement, cells are treated with antimycin (ETC inhibitor) and isoproterenol (β-adrenoreceptor agonist).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com