AB150674

Iron Stain Kit (Prussian Blue Stain)

Name: Iron Stain Kit (Prussian Blue Stain) (ab150674) | Abcam
Brand: Abcam Limited
SKU: AB150674
Price: 150 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(72 Publications)

Iron Stain Kit (Prussian Blue stain) ab150674 is used to stain for ferric iron in tissues, blood smears, or bone marrow smears.

- Suitable for Formalin-Fixed Paraffin-Embedded sections
- Cited in over 40 publications

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iron Stain Kit (Prussian Blue Stain) (AB150674)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iron Stain Kit (Prussian Blue Stain) (AB150674)

ab150674 Iron Stain Kit staining formalin-fixed-paraffin embedded human liver.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iron Stain Kit (Prussian Blue Stain) (AB150674)

ab150674 (Iron stain) staining iron deposits (blue) in formalin fixed paraffin embedded normal human liver, counterstained with nuclear fast red (pink).

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iron Stain Kit (Prussian Blue Stain) (AB150674)

Staining of Human Kidney using ab150674 - Iron Stain Kit.

Key facts

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Product details

How the assay works

This iron stain kit is based on the Prussian Blue stain reaction in which ionic iron reacts with acid ferrocyanide producing a blue color. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis.

Iron Stain Kit (Prussian Blue Stain) protocol summary:

- deparaffinize sections if necessary and rehydrate
- incubate for 3 min in iron stain (mix of potassium ferrocyanide and hydrochloric acid)
- rinse in water
- counterstain in nuclear fast red
- rinse in water
- dehydrate in alcohol and mount

Staining Interpretation

Iron Bright Blue
Nuclei Red
Background Pink
Control Tissue: Spleen, Bone Marrow

For Colloidal Iron, we recommend Colloidal Iron Stain Kit (Mucin Stain) ab150664.

How other researchers are using

Iron Stain Kit (Prussian Blue stain) has been used in a variety of sample type including:
- Intestinal epithelial cell line IEC-6 1
- Human endothelial progenitor cell 2
- Mouse ischemic retinas

References:
1 - Feng et al. 2023; 2 - Shin H et al. 2022; 3 - Dvoriantchikova et al. 2022.

Related and recommended products

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Other Notes

Please note, the 125 mL selling size, was previously described as 100 Test. There has been no change to the physical product.

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What's included?

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Properties and storage information

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Ambient

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Iron is a chemical element essential for various metabolic processes. It functions as a cofactor for proteins involved in oxygen transport and electron transfer. Iron exists in several forms including ferritin and hemosiderin and has a molecular mass of approximately 55.85 dalton. In human bodies iron is expressed mainly in red blood cells liver muscle tissues and is stored in ferritin complexes. For histological studies techniques such as iron staining and Perls Prussian blue are used to visualize iron in tissues. Additionally assays of iron and iron TIBC (Total Iron Binding Capacity) measure iron levels and binding affinity.

Biological function summary

Iron plays a role in cellular respiration DNA synthesis and electron transport. It is essential in hemoglobin within red blood cells and myoglobin in muscles where it binds and transports oxygen. Iron is also part of the iron-sulfur cluster and heme complexes important for electron exchange in cells. The blue stain used in histology highlights areas rich in iron aiding in diagnosis and research. You can assess iron availability and transport through tests like iron + TIBC.

Pathways

Iron is integral to oxygen transport and mitochondrial electron transport chain regulation. It is important in erythropoiesis and energy production. Iron works with proteins like transferrin and ferritin important for maintaining iron homeostasis. Cytochrome oxidase a heme-containing enzyme plays an essential role in electron transport linked with iron. Iron is also vital in modulating cellular oxidative stress pathways which help in managing reactive oxygen species.

Abnormal iron metabolism leads to conditions like anemia and hemochromatosis. In anemia deficiency of iron results in reduced oxygen transport due to low hemoglobin levels. Hemochromatosis on the other hand involves iron overload damaging organs like the liver and heart. The balance of iron is regulated by proteins including transferrin and ferritin which maintain necessary levels for cellular function and prevent toxic accumulation. The use of iron staining in histology assists in identifying iron-related pathologies.

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Product protocols

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Target data

See full target information Ferric cation

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Publications (72)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5420 PubMed40595589

2025

Normothermic human kidney preservation drives iron accumulation and ferroptosis.

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Marlon J A de Haan,Marleen E Jacobs,Annemarie M A de Graaf,Roan H van Scheppingen,Rico J E Derks,Dorottya K de Vries,Jesper Kers,Ian P J Alwayn,Cees van Kooten,Elena Sánchez-López,Martin Giera,Marten A Engelse,Ton J Rabelink

Pharmaceuticals (Basel, Switzerland) 18: PubMed40283982

2025

Treatment with Manganese Porphyrin, MnTnBuOE-2-PyP, Suppressed the Activation of Macrophages in a Mouse Intracerebral Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Zhang,Jie Cao,Ivan Spasojevic,Miriam Treggiari,Huaxin Sheng

NPJ Parkinson's disease 11:72 PubMed40216790

2025

Nigrostriatal iron accumulation in the progression of Parkinson's disease.

Applications

Unspecified application

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Unspecified reactive species

M López-Aguirre,T Balzano,M H G Monje,N Esteban-García,R Martínez-Fernández,N L Del Rey,M Ciorraga,A Sánchez-Ferro,I Trigo-Damas,J Blesa,J A Obeso,J A Pineda-Pardo

Theranostics 15:2883-2902 PubMed40083938

2025

7-nm Mn ZnFeO superparamagnetic iron oxide nanoparticle (SPION): a high-performance theranostic for MRI and hyperthermia applications.

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Joo Young Lee,Yi Rang Na,Chul Min Na,Pyung Won Im,Hyung Woo Park,Min Kyu Kim,Yona Kim,Ji Hyeon You,Dong Su Kang,Hyo Eun Moon,Hye Ran Park,Min Gyu Kim,Pilhan Kim,Sung Hye Park,Hye Won Youn,Young Don Son,Yasushi Takemura,Chang Won Song,Daishun Ling,Yuanzhe Piao,Sun Ha Paek

Nutrition & diabetes 15:6 PubMed39987125

2025

ZHX2 inhibits diabetes-induced liver injury and ferroptosis by epigenetic silence of YTHDF2.

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Wei Meng,Linghua Li

ACS omega 10:3535-3550 PubMed39926549

2025

Macrophage Responses to Multicore Encapsulated Iron Oxide Nanoparticles for Cancer Therapy.

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Unspecified application

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Sarah Kraus,Shir Arbib,Pazit Rukenstein,Irit Shoval,Raz Khandadash,Ofer Shalev

Scientific reports 15:2029 PubMed39815002

2025

MRI-guided focused ultrasound for treating Parkinson's disease with human mesenchymal stem cells.

Applications

Unspecified application

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Unspecified reactive species

Sheng-Kai Wu,Chia-Lin Tsai,Aisha Mir,Kullervo Hynynen

Physiological reports 12:e70139 PubMed39610053

2024

Western diet promotes the progression of metabolic dysfunction-associated steatotic liver disease in association with ferroptosis in male mice.

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Nicole Maddie,Nefia Chacko,David Matatov,Maria Alicia Carrillo-Sepulveda

Radiology advances 1:umae024 PubMed39574515

2024

Magnetic particle imaging enables nonradioactive quantitative sentinel lymph node identification: feasibility proof in murine models.

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Olivia C Sehl,Kelvin Guo,Abdul Rahman Mohtasebzadeh,Petrina Kim,Benjamin Fellows,Marcela Weyhmiller,Patrick W Goodwill,Max Wintermark,Stephen Y Lai,Paula J Foster,Joan M Greve

Heliyon 10:e39607 PubMed39506961

2024

Brain-targeting liposome-based gene delivery exacerbates soluble amyloid-β accumulation in mice.

Applications

Unspecified application

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Unspecified reactive species

Ni Wang,Tammee M Parsons,Yingxue Ren,Yining Pan,Aishe Kurti,Skylar C Starling,Chinenye Muolokwu,Jagdish Singh,Takahisa Kanekiyo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Iron Stain Kit (Prussian Blue Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iron Stain Kit (Prussian Blue Stain) (AB150674)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iron Stain Kit (Prussian Blue Stain) (AB150674)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Iron Stain Kit (Prussian Blue Stain) (AB150674)

Key facts

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Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (72)

Product promise