Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples.
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- Nature communications 14:43602023Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShubhi Srivastava,Priyanka Gajwani,Jordan Jousma,Hiroe Miyamoto,Youjeong Kwon,Arundhati Jana,Peter T Toth,Gege Yan,Sang-Ging Ong,Jalees RehmanPubMed 37468519
- Nature cancer 3:852-8652022Limited nutrient availability in the tumor microenvironment renders pancreatic tumors sensitive to allosteric IDH1 inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAli Vaziri-Gohar,Joel Cassel,Farheen S Mohammed,Mehrdad Zarei,Jonathan J Hue,Omid Hajihassani,Hallie J Graor,Yellamelli V V Srikanth,Saadia A Karim,Ata Abbas,Erin Prendergast,Vanessa Chen,Erryk S Katayama,Katerina Dukleska,Imran Khokhar,Anthony Andren,Li Zhang,Chunying Wu,Bernadette Erokwu,Chris A Flask,Mahsa Zarei,Rui Wang,Luke D Rothermel,Andrea M P Romani,Jessica Bowers,Robert Getts,Curtis Tatsuoka,Jennifer P Morton,Ilya Bederman,Henri Brunengraber,Costas A Lyssiotis,Joseph M Salvino,Jonathan R Brody,Jordan M WinterPubMed 35681100
- Communications biology 5:4672022Mitochondrial TrxR2 regulates metabolism and protects from metabolic disease through enhanced TCA and ETC function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE Sandra Chocron,Kennedy Mdaki,Nisi Jiang,Jodie Cropper,Andrew M PickeringPubMed 35577894
- Cell reports 39:1107192022Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin Ming-Chin Lee,Adrian A Achuthan,David P De Souza,Tanya J Lupancu,Katrina J Binger,Man K S Lee,Yangsong Xu,Malcolm J McConville,Nicole A de Weerd,Dragana Dragoljevic,Paul J Hertzog,Andrew J Murphy,John A Hamilton,Andrew J FleetwoodPubMed 35443173
- Signal transduction and targeted therapy 6:3752021The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXujun Liu,Wenzhe Si,Lin He,Jianguo Yang,Yani Peng,Jie Ren,Xiaoping Liu,Tong Jin,Huajing Yu,Zihan Zhang,Xiao Cheng,Wenting Zhang,Lu Xia,Yunchao Huang,Yue Wang,Shumeng Liu,Lin Shan,Yu Zhang,Xiaohan Yang,Haixia Li,Jing Liang,Luyang Sun,Yongfeng ShangPubMed 34728602
- Journal of neurovirology 27:367-3782021TCA cycle metabolic compromise due to an aberrant S-nitrosoproteome in HIV-associated neurocognitive disorder with methamphetamine use.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaschalis-Thomas Doulias,Tomohiro Nakamura,Henry Scott,Scott R McKercher,Abdullah Sultan,Amanda Deal,Matthew Albertolle,Harry Ischiropoulos,Stuart A LiptonPubMed 33876414
- Cell stem cell 27:748-764.e42020Nicotinamide Metabolism Mediates Resistance to Venetoclax in Relapsed Acute Myeloid Leukemia Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCourtney L Jones,Brett M Stevens,Daniel A Pollyea,Rachel Culp-Hill,Julie A Reisz,Travis Nemkov,Sarah Gehrke,Fabia Gamboni,Anna Krug,Amanda Winters,Shanshan Pei,Annika Gustafson,Haobin Ye,Anagha Inguva,Maria Amaya,Mohammad Minhajuddin,Diana Abbott,Michael W Becker,James DeGregori,Clayton A Smith,Angelo D'Alessandro,Craig T JordanPubMed 32822582
- Cell 180:1178-1197.e202020Aralar Sequesters GABA into Hyperactive Mitochondria, Causing Social Behavior Deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandros K Kanellopoulos,Vittoria Mariano,Marco Spinazzi,Young Jae Woo,Colin McLean,Ulrike Pech,Ka Wan Li,J Douglas Armstrong,Angela Giangrande,Patrick Callaerts,August B Smit,Brett S Abrahams,Andre Fiala,Tilmann Achsel,Claudia BagniPubMed 32200800
- Metabolic engineering communications 9:e000972019Application of a genome-scale model in tandem with enzyme assays for identification of metabolic signatures of high and low CHO cell producers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCyrielle Calmels et. al.PubMed 31720213
- Cell reports 28:218-230.e72019Nitric Oxide Modulates Metabolic Remodeling in Inflammatory Macrophages through TCA Cycle Regulation and Itaconate Accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJade D Bailey,Marina Diotallevi,Thomas Nicol,Eileen McNeill,Andrew Shaw,Surawee Chuaiphichai,Ashley Hale,Anna Starr,Manasi Nandi,Elena Stylianou,Helen McShane,Simon Davis,Roman Fischer,Benedikt M Kessler,James McCullagh,Keith M Channon,Mark J CrabtreePubMed 31269442
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Enzyme activity
- Assay time
45m
- Sensitivity
> 0.01 mU/well
Alternative names
Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, IDH1
What's included?
Recommended products
Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples.
Alternative names
Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, IDH1
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Enzyme activity
- Assay time
45m
- Assay Platform
Microplate reader
- Sensitivity
> 0.01 mU/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (λ = 450 nm) with detection sensitivity as low as 0.01 mU.
Visit our FAQs page for tips and troubleshooting.
Isocitrate dehydrogenase assay protocol summary:
- add samples and standards to wells
- incubate for 3 min and analyze with microplate reader
- incubate for 30 min - 2 hr and analyze again
This product is manufactured by BioVision, an Abcam company and was previously called K756 Isocitrate Dehydrogenase Activity Colorimetric Assay Kit. K756-100 is the same size as the 100 test size of ab102528.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Isocitrate Dehydrogenase often referred to as IDH includes multiple isoforms such as IDH1 and IDH2. These enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG) producing NADPH from NADP+ in the process. IDH1 weighs approximately 46 kDa and is located in the cytoplasm and peroxisomes while IDH2 operates in the mitochondria. Both isoforms of IDH are essential in cellular metabolism contributing to the citric acid cycle.
- Biological function summary
Isocitrate conversion catalyzed by IDH maintains the balance of cellular NADP+/NADPH which is important for protecting cells from oxidative stress. NADPH acts as a reducing agent in several anabolic reactions such as fatty acid and nucleotide synthesis. IDH does not function as part of a large complex but its action significantly impacts redox homeostasis within cells.
- Pathways
IDH plays a critical role in the citric acid cycle converting isocitrate to α-KG an important intermediate. This reaction connects to multiple metabolic pathways that influence energy production. Furthermore the production of α-KG links IDH activity with related proteins like α-Ketoglutarate dehydrogenase and glutamate dehydrogenase which further process metabolic intermediates in the cycle.
- Associated diseases and disorders
Mutations in the IDH1 and IDH2 genes link to various cancers such as glioma and acute myeloid leukemia (AML). These mutations often cause a neomorphic enzyme activity producing 2-hydroxyglutarate instead of α-KG which disrupts cellular metabolism and contributes to oncogenesis. The abnormal 2-hydroxyglutarate production interrelates with proteins involved in epigenetic regulation affecting DNA and histone methylation states which are frequently altered in cancerous cells.
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2 product images
Functional Studies - Isocitrate Dehydrogenase Assay Kit (Colorimetric) (ab102528)
Isocitrate Dehydrogenase detection in Bovine Liver Extraction Sample using ab102528
Functional Studies - Isocitrate Dehydrogenase Assay Kit (Colorimetric) (ab102528)
Example of NADH and NADPH Standard Curve obtained using ab102528
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