JC-1 - Mitochondrial Membrane Potential Assay Kit

JC1- Mitochondrial Membrane Potential Assay Kit ab113850 contains tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in energized mitochondria.

At low concentrations (due to low mitochondrial membrane potential), JC-1 is predominantly a monomer that yields green fluorescence with emission of 530±15 nm.

At high concentrations (due to high mitochondrial membrane potential), the dye aggregates yielding a red to orange colored emission (590±17.5 nm).

Therefore a decrease in the aggregate fluorescent count is indicative of depolarization whereas an increase is indicative of hyperpolarization.

The JC-1 staining protocol is very simple:
- wash cells in dilution buffer or PBS
- add JC solution
- incubate for 30 min at 37°C for suspension cells, or 10 min for adherent cells
- wash cells with dilution buffer
- treat cells as desired for experimental plan
- analyze on a fluorescent microplate reader

The aggregate dye can be excited at 535 nm, the monomer and aggregate together at 475 nm.

Review our to learn more about our other , and assay kits. Review the to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.