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JC1- Mitochondrial Membrane Potential Assay Kit ab113850 contains tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in energized mitochondria.

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Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay time

1h

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Shipping To:
United States

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What's included?

100 Test
Components
50mM FCCP (in DMSO)
1 x 10 µL
DMSO (cell culture tested)
1 x 1 mL
Dilution Buffer (10X, sterile)
1 x 10 mL
JC-1 (lyophilized)
1 x 500 µg

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JC1- Mitochondrial Membrane Potential Assay Kit ab113850 contains tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in energized mitochondria.

Is this your first time purchasing this product? Get 20% off one unit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, and Australia.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay time

1h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

JC1- Mitochondrial Membrane Potential Assay Kit ab113850 contains tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in energized mitochondria.

At low concentrations (due to low mitochondrial membrane potential), JC-1 is predominantly a monomer that yields green fluorescence with emission of 530±15 nm.

At high concentrations (due to high mitochondrial membrane potential), the dye aggregates yielding a red to orange colored emission (590±17.5 nm).

Therefore a decrease in the aggregate fluorescent count is indicative of depolarization whereas an increase is indicative of hyperpolarization.

The JC-1 staining protocol is very simple:
- wash cells in dilution buffer or PBS
- add JC solution
- incubate for 30 min at 37°C for suspension cells, or 10 min for adherent cells
- wash cells with dilution buffer
- treat cells as desired for experimental plan
- analyze on a fluorescent microplate reader

The aggregate dye can be excited at 535 nm, the monomer and aggregate together at 475 nm.

Review our to learn more about our other , and assay kits. Review the to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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