Skip to main content

JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) ab112134 enables researchers to analyze a JC-10 assay with a microplate reader.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay time

1h

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Images

What's included?

5 x 96 Tests
Components
100X JC-10 in DMSO
1 x 250 µL
Assay Buffer A
1 x 25 mL
Assay Buffer B
1 x 25 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) ab112134 enables researchers to analyze a JC-10 assay with a microplate reader.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay time

1h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) ab112134 enables researchers to analyze a JC-10 assay with a microplate reader. The JC-10 assay provides the most robust assay method for monitoring mitochondria membrane potential changes.

This mitochondrial membrane potential assay protocol is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye. In normal cells, JC-10 concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. However, in apoptotic and necrotic cells, JC-10 diffuses out of mitochondria. It changes to monomeric form and stains cells in green fluorescence.

Although JC-1 is widely used in many labs, its poor water solubility causes great inconvenience. Even at 1 μM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 is developed to be a superior alternative to JC-1 when high dye concentration is desired. Compared to JC-1, JC-10 has much better water solubility. JC-10 is capable of selectively entering mitochondria, and reversibly changes its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e. emission of JC-10 monomeric form) to 570 nm (i.e. emission of J-aggregate form). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized.

In normal cells, JC-10 concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. However, in apoptotic and necrotic cells, JC-10 exists in monomeric form and stains cells green. The green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its use in fluorescence microplate platform, it can also be used in fluorescence imaging and flow cytometry.

A microplate reader with bottom-reading mode is essential to perform this assay. If you would like to use JC-10 on a flow cytometer, we recommend . **Related assays** Review the to learn about kits to perform a , and . Review the to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com