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AB113459

KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric)

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(2 Publications)

KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines.

View Alternative Names

AOF2, KDM1, KIAA0601, LSD1, KDM1A, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A

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Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (AB113459)
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Supplier Data

Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (AB113459)

Demonstration of inhibitory effects of an KDM1/LSD1 inhibitor detected by ab113459. [LSD1 concentration : 200 ng/well]

Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (AB113459)
  • FuncS

Supplier Data

Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (AB113459)

Demonstration of high sensitivity of KDM1 / LSD1 activity assay achieved by using recombinant KDM1 / LSD1 with ab113459.

Key facts

Detection method

Colorimetric

Assay type

Enzyme activity

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.

KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459) is designed for the easy and fast colorimetric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

This kit may cross react with LSD2.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab113459-96Test", "assetComponentDetails": [ { "size":"1 x 120 µL", "name":"LSD1 Substrate, 50 µg/mL", "number":"AB113459-CMP10" }, { "size":"1 x 8 mL", "name":"LSD1 Assay Buffer", "number":"AB113459-CMP07" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113459-CMP01" }, { "size":"1 x 10 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113459-CMP04" }, { "size":"1 x 28 mL", "name":"10X Wash Buffer", "number":"AB113459-CMP03" }, { "size":"1 x 20 µL", "name":"LSD1 Assay Standard, 50 µg/mL", "number":"AB113459-CMP08" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113459-CMP02" }, { "size":"1 x 12 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113459-CMP05" }, { "size":"1 x 40 µL", "name":"LSD1 Inhibitor Tranylcypromine, 1 mM", "number":"AB113459-CMP09" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB113459-CMP11" }, { "size":"1 x 10 mL", "name":"Developer Solution", "number":"AB113459-CMP06" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab113459-48Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113459-CMP02", "productcode":"" }, { "size":"1 x 6 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113459-CMP05", "productcode":"" }, { "size":"1 x 5 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113459-CMP04", "productcode":"" }, { "size":"1 x 10 µL", "name":"LSD1 Assay Standard, 50 µg/mL", "number":"AB113459-CMP08", "productcode":"" }, { "size":"1 x 60 µL", "name":"LSD1 Substrate, 50 µg/mL", "number":"AB113459-CMP10", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113459-CMP01", "productcode":"" }, { "size":"1 x 14 mL", "name":"10X Wash Buffer", "number":"AB113459-CMP03", "productcode":"" }, { "size":"1 x 4 mL", "name":"LSD1 Assay Buffer", "number":"AB113459-CMP07", "productcode":"" }, { "size":"1 x 20 µL", "name":"LSD1 Inhibitor Tranylcypromine, 1 mM", "number":"AB113459-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"Developer Solution", "number":"AB113459-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB113459-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed : 15620353, PubMed : 15811342, PubMed : 16079794, PubMed : 16079795, PubMed : 16140033, PubMed : 16223729, PubMed : 27292636). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed : 15620353, PubMed : 15811342, PubMed : 16079794, PubMed : 21300290). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed : 15620353, PubMed : 20389281, PubMed : 21300290, PubMed : 23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed : 16079794, PubMed : 16140033, PubMed : 16885027, PubMed : 21300290, PubMed : 23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed : 16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 (PubMed : 29691401). Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 (PubMed : 29358331). Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed : 16079794, PubMed : 16140033). Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 (PubMed : 20562920, PubMed : 27292636). Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed : 20389281).
See full target information KDM1A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:6282 PubMed34725362

2021

Nuclear S-nitrosylation impacts tissue regeneration in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Gianfranco Matrone,Sung Yun Jung,Jong Min Choi,Antrix Jain,Hon-Chiu Eastwood Leung,Kimal Rajapakshe,Cristian Coarfa,Julie Rodor,Martin A Denvir,Andrew H Baker,John P Cooke

Neuropsychobiology 81:116-126 PubMed34515196

2021

The Effect of a Probiotic Complex on the Gut-Brain Axis: A Translational Study.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Nobile,Silvana Giardina,Francesco Puoci
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