KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460)

KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines.

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Alternative names=Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A, KDM1A, AOF2, KDM1, KIAA0601, LSD1

Detection method: Fluorescent
Assay type: Enzyme activity
Reactive species: Mouse, Human, Plants
Assay time: 3h

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Target data

KDM1A

What's included?

96 Test

Components

10X Wash Buffer

1 x 28 mL

8-Well Assay Strips (with Frame)

12 x 1 Unit

Adhesive Covering Film

1 x 1 Unit

Capture Antibody, 1000 µg/mL

1 x 10 µL

Detection Antibody, 400 µg/mL

1 x 12 µL

Fluoro Developer

1 x 20 µL

Fluoro Diluter

1 x 8 mL

Fluoro Enhancer

1 x 20 µL

LSD1 Assay Buffer

1 x 8 mL

LSD1 Assay Standard, 50 µg/mL

1 x 20 µL

LSD1 Inhibitor Tranylcypromine, 1 mM

1 x 40 µL

LSD1 Substrate, 50 µg/ml

1 x 120 µL

Key facts

Detection method: Fluorescent
Assay type: Enzyme activity
Reactive species: Mouse, Human, Plants
Assay time: 3h
Assay Platform: Microplate reader

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: Multi
Appropriate long-term storage conditions: Multi
Storage information: Please refer to protocols

Notes

KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3 (meK4), a specific tag for epigenetic transcriptional activation.

KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (ab113460) is designed for the easy and fast fluorometric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

This kit may cross react with LSD2.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric)

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