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KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation.

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Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (AB113461), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Assay type
Enzyme activity
Reactive species
Mouse, Human
Assay time
3h

Associated Products

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Target data

Function

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:26741168). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Isoform 2. Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.

Alternative names

JHDM3A, JMJD2, JMJD2A, KIAA0677, KDM4A, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A, [histone H3]-trimethyl-L-lysine(36) demethylase 4A, [histone H3]-trimethyl-L-lysine(9) demethylase 4A

What's included?

96 Test
Components
10X Wash Buffer
1 x 28 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Adhesive Covering Film
1 x 1 Unit
Capture Antibody, 1000 µg/mL
1 x 10 µL
Co-factor 1
1 x 60 µL
Co-factor 2
1 x 60 µL
Co-factor 3
1 x 60 µL
Detection Antibody, 400 µg/mL
1 x 12 µL
Developer Solution
1 x 10 mL
JMJD2 Assay Buffer
1 x 8 mL
JMJD2 Assay Standard, 50 µg/mL
1 x 20 µL
JMJD2 Substrate, 50 µg/mL
1 x 120 µL
Stop Solution
1 x 10 mL

Recommended products

KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation.

Key facts

Detection method
Colorimetric
Assay type
Enzyme activity
Reactive species
Mouse, Human
Assay time
3h
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.

KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461) is designed for the easy and fast colorimetric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

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1 product image

  • Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461), expandable thumbnail

    Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461)

    Demonstration of high sensitivity of the KDM4/JMJD2 activity assay achieved by using recombinant KDM4/JMJD2 with ab113461.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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