JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB113461

KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(2 Publications)

KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation.

View Alternative Names

JHDM3A, JMJD2, JMJD2A, KIAA0677, KDM4A, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A, [histone H3]-trimethyl-L-lysine(36) demethylase 4A, [histone H3]-trimethyl-L-lysine(9) demethylase 4A

1 Images
Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (AB113461)
  • FuncS

Supplier Data

Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (AB113461)

Demonstration of high sensitivity of the KDM4/JMJD2 activity assay achieved by using recombinant KDM4/JMJD2 with ab113461.

Key facts

Detection method

Colorimetric

Assay type

Enzyme activity

Assay time

3h

Assay Platform

Microplate reader

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.

KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461) is designed for the easy and fast colorimetric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab113461-96Test", "assetComponentDetails": [ { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113461-CMP01" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113461-CMP05" }, { "size":"1 x 12 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113461-CMP08" }, { "size":"1 x 28 mL", "name":"10X Wash Buffer", "number":"AB113461-CMP06" }, { "size":"1 x 120 µL", "name":"JMJD2 Substrate, 50 µg/mL", "number":"AB113461-CMP12" }, { "size":"1 x 60 µL", "name":"Co-factor 1", "number":"AB113461-CMP02" }, { "size":"1 x 8 mL", "name":"JMJD2 Assay Buffer", "number":"AB113461-CMP10" }, { "size":"1 x 10 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113461-CMP07" }, { "size":"1 x 20 µL", "name":"JMJD2 Assay Standard, 50 µg/mL", "number":"AB113461-CMP11" }, { "size":"1 x 60 µL", "name":"Co-factor 3", "number":"AB113461-CMP04" }, { "size":"1 x 60 µL", "name":"Co-factor 2", "number":"AB113461-CMP03" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB113461-CMP13" }, { "size":"1 x 10 mL", "name":"Developer Solution", "number":"AB113461-CMP09" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab113461-48Test", "assetComponentDetails": [ { "size":"1 x 60 µL", "name":"JMJD2 Substrate, 50 µg/mL", "number":"AB113461-CMP12", "productcode":"" }, { "size":"1 x 5 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113461-CMP07", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 2", "number":"AB113461-CMP03", "productcode":"" }, { "size":"1 x 4 mL", "name":"JMJD2 Assay Buffer", "number":"AB113461-CMP10", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113461-CMP01", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 1", "number":"AB113461-CMP02", "productcode":"" }, { "size":"1 x 14 mL", "name":"10X Wash Buffer", "number":"AB113461-CMP06", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 3", "number":"AB113461-CMP04", "productcode":"" }, { "size":"1 x 6 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113461-CMP08", "productcode":"" }, { "size":"1 x 10 µL", "name":"JMJD2 Assay Standard, 50 µg/mL", "number":"AB113461-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113461-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB113461-CMP13", "productcode":"" }, { "size":"1 x 5 mL", "name":"Developer Solution", "number":"AB113461-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed : 26741168). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.. Isoform 2. Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
See full target information KDM4A
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684884

2024

KDM4 Regulates the Glycolysis of Hemocytes in the Immune Priming of .

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Zhao,Xue Qiao,Simiao Yu,Yuhao Jin,Jixiang Niu,Jie Li,Yingmei Xu,Yuehong Yang,Lingling Wang,Linsheng Song

The Journal of biological chemistry 298:102536 PubMed36174675

2022

Intermittent hypoxia enhances the expression of hypoxia inducible factor HIF1A through histone demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Chloe-Anne Martinez,Yannasittha Jiramongkol,Neha Bal,Imala Alwis,Polina E Nedoboy,Melissa M J Farnham,Mark D White,Peter A Cistulli,Kristina M Cook
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Proteins & Peptides

AB198094

Recombinant human KDM4C / GASC1 / JMJD2C protein (Tagged)

Functional Studies - Recombinant human KDM4C / GASC1 / JMJD2C protein (Tagged) (AB198094)

  • 0
  • 0
Assay Kits

AB113460

KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric)

Functional Studies - KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric) (AB113460)

  • 0
  • 0
Assay Kits

AB113462

KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric)

Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (AB113462)

  • 0
  • 0
Primary Antibodies

AB22758

Anti-Nucleolin antibody

BOND RX™ Validated

Western blot - Anti-Nucleolin antibody (AB22758)

  • 4
  • 8

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com